Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of soybean (Glycine max (L.) Merr.) in North America, since it was first found i...

Marker-assisted backcross breeding program was anticipated to introgress bacterial blight (BB) resistance genes into an elite fine-grain rice cultivar VGD 1. Pyramided with three (xa5, Xa13, Xa21), ADT 55 the donor parent. A parental polymorphism survey executed between VGD1 and using genome-wide simple sequence repeats (SSR) also called microsatellite markers, identify polymorphic markers. Dis...

Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from associ...

Molecular marker-assisted selection (MAS) is currently being used to accelerate the development of rice cultivars with improved cooking quality and blast resistance at the University of Arkansas Rice Research and Extension Center (UA RREC) in Stuttgart. A co-dominant simple sequence repeat (SSR) marker, RM 190, is used to predict cooking quality of the milled grain, and a new automation-friendl...

Hydrangea (Hydrangea macrophylla) is an important ornamental crop that has been cultivated for more than 300 years. Despite the economic importance, genetic studies hydrangea have limited by lack of resources. Genetic linkage maps and subsequent trait mapping are essential tools to identify make markers available marker-assisted breeding. A transcriptomic study was performed on two cultivars, V...

Introduction More than 1000 morphological markers have been identified in barley (Franckowiak and Lundqvist 2002). These phenotypic traits were observed as spontaneous or artificially generated mutants in a wide range of cultivars over decades of research. Traits identified with alternate alleles include plant height, spike morphology, and seed size, among others. Approximately 275 of these mar...

The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene (Rf) to restore fertility in another are indispensable for the development of hybrid varieties. To genotype rice lines at the restoring fertility (Rf) loci, 38 lines were crossed with a sterile tester (rfrf) line. Pollen fertility test was performed to identify sterile and fertile F1 hybrids. Seven lines were ...

Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...