نتایج جستجو برای: spindle

تعداد نتایج: 26260  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1993
C J Hogan H Wein L Wordeman J M Scholey K E Sawin W Z Cande

Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody local...

Journal: :Automatika 2022

SSD (synchronized switch damping) is used for vibration control of the motorized spindle based on piezoelectric stack. Moreover, inspired by self-powered SSDI, a SSDV circuit was designed to overcome disadvantages requiring readjusting parameters and sensor re-positioning SSDI damping inductor) voltage source). A simulation an experimental were built, results show performance better than more f...

Journal: :International journal of applied dental sciences 2022

Sarcomatoid carcinoma is a type of spindle cell neoplasm. These varieties neoplasms comprise diverse collection benign and malignant neoplasms. carcinoma, also known by various other names such as Spindle Lane’s tumor, to name few, an exceedingly rare aggressive variant squamous 3, 7, 8 The variation in its nomenclature reflects the divergent interpretation sarcomatoid component reactive or neo...

Journal: :The Journal of Cell Biology 1996
H Wein M Foss B Brady W Z Cande

We have identified an 80-kD protein that is involved in mitotic spindle elongation in the diatom Cylindrotheca fusiformis. DSK1 (Diatom Spindle Kinesin 1) was isolated using a peptide antibody raised against a conserved region in the motor domain of the kinesin superfamily. By sequence homology, DSK1 belongs to the central motor family of kinesin-related proteins. Immunoblots using an antibody ...

Journal: :Current Biology 2000
Marie-Hélène Verlhac Christophe Lefebvre Philippe Guillaud Pascale Rassinier Bernard Maro

In both vertebrates and invertebrates, meiotic divisions in oocytes are typically asymmetric, resulting in the formation of a large oocyte and small polar bodies. The size difference between the daughter cells is usually a consequence of asymmetric positioning of the spindle before cytokinesis. Spindle movements are often related to interactions between the cell cortex and the spindle asters [1...

Journal: :Journal of cell science 1992
R J Leslie

To examine the relative roles of chromosomes, spindle poles and microtubules in the formation of the metaphase spindle and metakinesis, I have experimentally placed an extra centrosome-free pronucleus close to a forming bipolar spindle in a living cell. The chromosomes from the extra nucleus induce the formation of an extra half-spindle from one pole of the otherwise normal bipolar spindle with...

2012
Ana M. Rojas Anna Santamaria Rainer Malik Thomas Skøt Jensen Roman Körner Ian Morilla David de Juan Martin Krallinger Daniel Aaen Hansen Robert Hoffmann Jonathan Lees Adam Reid Corin Yeats Anja Wehner Sabine Elowe Andrew B. Clegg Søren Brunak Erich A. Nigg Christine Orengo Alfonso Valencia Juan A. G. Ranea

The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integra...

Journal: :American journal of physiology. Cell physiology 2017
Ashley L Severance Keith E Latham

Oocyte meiotic spindles are associated with spindle-enriched mRNAs, phosphorylated ribosome protein S6, and phosphorylated variants of the key translational regulator, eukaryotic translation initiation factor 4E-binding protein 1 (eIF4E-BP1), consistent with translational control of localized mRNAs by eIF4E-BP1 in facilitating spindle formation and stability. Using specific kinase inhibitors, w...

Journal: :The Biochemical journal 2013
Yuko Iwakiri Sachiko Kamakura Junya Hayase Hideki Sumimoto

Bipolar spindle assembly in mitotic cells is a prerequisite to ensure correct alignment of chromosomes for their segregation to each daughter cell; spindle microtubules are tethered at plus ends to chromosomes and focused at minus ends to either of the two spindle poles. NuMA (nuclear mitotic apparatus protein) is present solely in the nucleus in interphase cells, but relocalizes during mitosis...

Journal: :Journal of cell science 2011
Nan Ma Sachihiro Matsunaga Akihiro Morimoto Gyosuke Sakashita Takeshi Urano Susumu Uchiyama Kiichi Fukui

Segregation of chromosomes during cell division requires correct formation of mitotic spindles. Here, we show that a scaffold attachment factor A (SAF-A), also known as heterogeneous nuclear ribonucleoprotein-U, contributes to the attachment of spindle microtubules (MTs) to kinetochores and spindle organization. During mitosis, SAF-A was localized at the spindles, spindle midzone and cytoplasmi...

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