Smnmary. Washed whole chloroplasts of Spinacia oleracea isolated and assayed in a tris (hydroxymethyl aminomethane)-HCl bu'ifered sucrose solution exhibited low dark CO.2 fixing activity, whereas washed whole chloroplasts isolated in the same buffer but assayed in that ibuffer without sucrose exh;ibited much greater dark CO2 'fixing activity. The lowered activity could be attributed to the impe...

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We have used the carotenoid biosynthesis inhibitor norflurazon to study the relationship between chloroplast and nuclear gene expression and the mechanisms by which plastid mRNA accumulation is regulated in response to photooxidative stress. By treating 4-week-old hydroponic spinach plants (Spinacea oleracea), we were able to determine the response at two distinct stages of chloroplast developm...

Ribulose-l,5-bisphosphate carboxylase/oxygenase activase often consists of two polypeptides that arise from alternative splicing of pre-mRNA. In this study recombinant versions of the spinach (Spinacea oleracea L.) 45and 41-kD forms of activase were analyzed for their response to temperature. The temperature optimum for ATP hydrolysis by the 45-kD form was 45”C, approximately 13°C higher than t...

The flavoprotein NADPH-ferredoxin oxidoreductase (EC 1.6.99.4) is a component of the photosynthetic electron-transport chain, catalysing the reduction of NADP+ with reduced ferredoxin (Shin et al., 1965). The enzyme also possesses activity as a NADPH diaphorase (Avron & Jagendorf, 1956), a transhydrogenase (Keister et a/., 1960) and NADPH-cytochrome f reductase (Zanetti & Forti, 1966) all three...

Objective(s) Portulaca oleracea L. is a herbaceous weed from portulacaceae family. It can be found in many parts of the world. Modern pharmacological studies have demonstrated that P. oleracea have antioxidant effects. The protective effect of aqueous and ethanolic extract of P. oleracea against cisplatin-induced renal toxicity was studied in rats. Materials and Methods Single intraperitonea...

Detection of phytoplasma classified in phylogenetic group 16SrI, ‘Ca. Phytoplasma asteris’, in the plants of interspecific genotypes of Brassica oleracea x Brassica napus and their back-cross toward B. oleracea (IW7.7xB1) is reported in Poland. The presence of phytoplasmas in leaves of plants showing failure of flower bud formation but not in the asymptomatic hybrids was demonstrated by polymer...

BACKGROUND The cabbage, Brassica oleracea var. capitata L., has a distinguishable phenotype within the genus Brassica. Despite the economic and genetic importance of cabbage, there is little genomic data for cabbage, and most studies of Brassica are focused on other species or other B. oleracea subspecies. The lack of genomic data for cabbage, a non-model organism, hinders research on its molec...

Please cite this article in press as: Pacheco ... Food Chemistry (2009), doi:10.1016/j.foo Açai fruit are native to the Amazon region of South America and two predominant species are commercially exported as fruit pulps for use in food and beverage applications. Detailed characterisation of the polyphenolic compounds present in the de-seeded fruits of Euterpe oleracea and Euterpe precatoria spe...