Plasma membrane isolated from rat sperm cells after incubation in vitro had a significantly lower cholesterol/phospholipid mole ratio when the medium contained serum albumin. Transfer of albumin-bound phospholipids to the membrane can largely account for this effect. The result is broadly consistent with a previously proposed model for albumin-induced destabilization of sperm membrane (capacita...

Background: Oxidative stress and decreased antioxidant levels have been projected as a potential factors involved in the pathophysiology of diverse male infertility types, including asthenospermia. The generation of reactive oxygen species (ROS) in the male reproductive tract has become an actual concern because of their probable noxious effects, at high levels, on physical properties of sperm ...

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 4...

Cervically inseminated cryopreserved ram spermatozoa have reduced fertility due to poor mucus-penetrating ability. This effect is ameliorated by the addition of 20% (v/v) seminal plasma (SP) to the phosphate-buffered saline (PBS) thawing medium. The aims of this study were to determine whether the impaired mucus penetration was due to alterations in the sperm motility and, if so, whether these ...

S PERMATOZOA from the caput, corpus and cauda epididymis and ejaculated sperm were analyzed for their phospholipid and phospholipid fatty acid content. Caput spermatozoa contained 16 and 34% more total phospholipid than sperm from the corpus and cauda epididymis, respectively. Caudal sperm were similar to ejaculated sperm in phospholipid content. Seminal plasma from ejaculated semen collected a...

OBJECTIVE To evaluate the effectiveness of coenzyme Q(10) treatment in improving semen quality in men with idiopathic infertility. DESIGN Placebo-controlled, double-blind randomized trial. SETTING Andrology Unit, Department of Internal Medicine, Polytechnic University of Marche, Italy. PATIENT(S) Sixty infertile patients (27-39 years of age) with the following baseline sperm selection cri...

The global proteome of sperm and seminal plasma of fertile stallions was investigated to determine whether associations with relative in vivo fertility exist. Seven stallions at stud in a commercial breeding station were collected throughout the breeding season and bred to a total of 164 mares to determine conception rates. On three occasions during the breeding season, raw semen was obtained f...

BACKGROUND Oxidative DNA damage is associated with male infertility. The aim of this study was to evaluate the oxidative DNA damage of sperm cells and blood leukocytes and to determine the levels of MDA and NO levels in seminal and blood plasma of idiopathic infertile men. MATERIAL AND METHODS The study enrolled 52 patients, including 30 infertile and 22 fertile men. MDA, NO, and 8-OHdG/106dG w...

BACKGROUND Semen analysis is the cornerstone in the evaluation of male (in)fertility. However, there are men with normal semen tests but with impaired fertilizing ability, as well as fertile men with poor sperm characteristics. Thus, there is rising interest to find novel parameters that will help to predict and define the functional capacity of spermatozoa. METHODS We examined whether there ...

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