Intracytoplasmic sperm injection (ICSI) is a promising assisted-fertilisation technique that may benefit women who have not become pregnant by in-vitro fertilisation (IVF) or subzonal insemination (SUZI) of oocytes. We have used ICSI to treat couples with infertility because of severely impaired sperm characteristics, and in whom IVF and SUZI had failed. Direct injection of a single spermatozoo...

PURPOSE The objective was to describe the results of the injection of immotile spermatozoa with flexible tails when only immotile spermatozoa are present in the semen sample. METHODS A retrospective study was conducted to analyze the procedure results for 10 couples who participated in our intracytoplasmic sperm injection program. The sperm tail was considered flexible when it moved up and do...

Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opport...

The growing popularity of vasectomy as a primary form of birth control has also created an increasing demand for vasectomy reversal (VR). The advent of IVF with intracytoplasmic sperm injection after sperm retrieval has given couples an alternative to traditional VR. Although VR remains the most cost-effective and natural way to conceive after vasectomy, competing interests and market pressures...

Background: The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized w...

One in six couples of reproductive age worldwide are affected at least once by some form of infertility. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are widely-available assisted reproductive technologies (ART). The identification and isolation of the most-motile sperm with DNA integrity are essential to IVF and ICSI, ultimately affecting treatment consequences and ...

Six experiments were conducted to determine the ability of exogenous estradiol-17/3 to increase numbers of sperm in the oviducts, uteri and cervices of inseminated estrous ewes. In a 2 x 3 factorial experiment, ewes were mated during either early or late estrus; ewes in each group were either not treated or were treated with estradiol by subcutaneous injection once, at 6 hr after mating (30 /ag...

objective: epididymo-orchitis (eo) potentially results in reduced fertility in up to 60% of affected patients. the anti-inflammatory effects of korean red ginseng (krg) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute eo. ma...

Spinal cord injury (SCI) occurs most often in young men at the peak of their reproductive health. The majority of men with SCI cannot father children naturally. Three major complications contribute to infertility in men with SCI: erectile dysfunction, ejaculatory dysfunction, and abnormal semen quality. Erectile dysfunction can be managed by regimens available to the general population, includi...

the effect of subcutaneous injections of vitamin c on the seminal characteristics of markhoz bucks (2–4-year-old) was studied. the bucks, trained to serve an artificial vagina, were randomly allotted into three equal groups (n = 4) and received daily either zero (1 ml normal saline; control group), or 20 (vitc20 group) or 40 (vitc40 group) mg per kg body weight vitamin c from july 06, 2006 to o...