tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Human assisted reproduction results have improved dramatically in the last 20 years, with lower complications for the patient as well as for the newborns. This requires a precise clinical diagnosis that will allow us to individualize the best-personalized treatment for each patient or couple. But still today, the basi...

BACKGROUND We evaluated the impact of testicular germ cell cancer (TGCC), its treatment and length of follow-up on sperm DNA integrity. METHODS In 96 TGCC patients, semen was collected at specific intervals until 5 years after treatment. Sperm DNA integrity was assessed by the sperm chromatin structure assay (SCSA, n = 193) and by the terminal deoxynucleotidyl transferase-mediated dUTP nick-e...

Raman Spectroscopy was evaluated as a non-invasive method of analysis of sperm DNA and the influence of UV irradiation on the sperm. The results show that Raman Spectroscopy, combined with multivariate analysis provide the reproducible and accurate information on DNA of sperm and the effect and location of damage.

The traditional semen analysis is a cornerstone in diagnosis and treatment of male fertility (World Health Organisation (WHO), 1999, 2010). The sperm parameters concentration, motility and morphology are, however, claimed to be poor predictors of a male ́s fertility status, this in natural conception (Bonde et al., 1998; Guzick et al., 2001) as well as in assisted reproductive techniques (ART) (...

Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay (SCSA) parameters, that is, DNA fragmentation index (%DFI) and high DNA sta...

Background: Sperm chromatin integrity assessment has been suggested as fertility predictor. The aim of this study was to examine the relationship between the results of sperm chromatin structure assay (SCSA) and the outcome of intrauterine insemination IUI. Materials and Methods: A total of 576 consecutive couples undergoing IUI were included and IUI was performed. SCSA results were expressed a...

Background: About 15% of male infertility is due to genital tract infection and inflammation, some them have no clinical symptoms, but manifested as leukocytospermia (LCS). Leukopenia will lead functional impairment sperm integrity damage morphology. A large amount reactive oxygen species (ROS) produced by leukocytes can nuclear DNA. The aim this study was investigate the correlation between le...

Background: Diabetes mellitus as a chronic disease can cause fertility problems in male human population. In fact, it exerts sever damages in testicular tissue and subsequently reduces total number of normal sperms and semen volume. Therefore, this study is designed to evaluate the protective effect of Equisetum arvense extract on sperm quality, DNA damage and maturation in diabetic male mice. ...

Background: Prolonged alcohol abuse in men can cause testosterone deficiency and shrinkage of the testicles (testicular atrophy). This can lead to impotence, sterility and ‘feminisation’. When alcohol is broken down in the body, it may also interfere with normal sperm structure and movement. This study was designed to determine the effects of administration of alcohol on sperm quality, sperm ma...