نتایج جستجو برای: sperm counting chamber
تعداد نتایج: 116074 فیلتر نتایج به سال:
This study examines the effect of varying several factors, both extrinsic and technology-dependent, on the reconstruction of human sperm trajectories and the derived kinematic measurements using videotapes and the Motion Analysis Celltrak/S instrument. In semen samples from normal healthy men, curvilinear (VCL) and straight line velocities (VSL) were found to increase 1.5-fold, and linearity (L...
The article describes a new proposal for quantitative melissopalynological analysis of pollen grains in 10 g bee honey, using a Bürker chamber for peripheral white blood cells counting. The research related to the development of the method and its algorithm are presented. Further investigations in certified laboratories are required to evaluate the repeatability and reproducibility of the metho...
Marine invertebrates exhibit both chemokinesis and chemotaxis phenomena, induced in most cases by the release of water-borne peptides or pheromones. In mollusks, several peptides released during egg-laying improve both male attraction and mating. Unlike other cephalopods, Octopus vulgaris adopts an indirect internal fertilization strategy. We here report on the identification and characterizati...
Nematode eggs were collected by mixing the faeces with a sugar solution, filling a flat-sided medicine bottle (100 ml), and allowing eggs to float and adhere to the upper surface for 30 min. After discarding the faeces, eggs hatched within 24 h at 20-30 degrees C. Each genus was counted in a counting chamber and the L1p.g. estimated. The morphology of L1 of Haemonchus, Teladorsagia, Trichostron...
Structural changes inside the head of Thyone sperm undergoing the acrosomal reaction were followed with a high-resolution, differential interference contrast (DIC) video microscope. The beating sperm, adhering by their midpiece to the cover slip of a wedge perfusion chamber, were activated by a calcium ionophore (20 microM A23187) suspended in sea water containing 50 mM excess CaCl2. Before act...
Introduction: Spinal cord injury (SCI) is a serious public health problem which seriously affects the victim, family, and even the society. Research studies have shown that 80% of SCI victims are men. In recent years, there have been extensive research works on the effect of SCI (acute and/or chronic) on fertility potential of sperm and spermatogenesis in laboratory animals. SCI may disturb the...
A tissue culture bilayer system that mimics some aspects of early alveolar infection by Mycobacterium tuberculosis was developed. This model incorporates human lung epithelial type II pneumocyte (A549) (upper chamber) and endothelial cell (lower chamber) layers separated by a microporous membrane. This construction makes it possible to observe and quantify the passage of bacteria through the tw...
Infertility affects approximately 15% of couples with equivalent male and female contribution. Absence of sperm in semen, referred to as azoospermia, accounts for 5-20% of male infertility cases and can result from pretesticular azoospermia, non-obstructive azoospermia (NOA), and obstructive azoospermia (OA). The current clinical methods of differentiating NOA cases from OA ones are indetermina...
OBJECTIVE To explore the possibility of using the L4 membrane filtration device for processing of spermatozoa for intrauterine insemination and other assisted reproductive techniques. DESIGN, SETTING, PATIENTS Semen samples from 15 patients attending an infertility clinic of a tertiary referral institution and from seven fertile donors were used for the study. MAIN OUTCOME MEASURES The effe...
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