A highly selective methacrylic based molecularly imprinted polymer (MIP) was synthesized and applied for the separation and the pre-concentration of salbutamol in urine samples. Spectrophotometric determination of salbutamol was achieved using 2,6-dichloroquinone chlorimide as colorimetric reagent. The detection limit of the method was ca. 13 ng mL in urine after pre-concentration of the sample...

Dynamic models of M-type Mira variables predict the occurrence of water " shells " , i.e. of zones of high H2O density and high H2O absorption inside the stellar atmosphere. The density, position and width of these shells is closely correlated with different types of two-component shapes of the intensity distribution on the disk in the H, K and L near-continuum bandpasses. We investigate these ...

The post-antibiotic effects of gentamicin and ciprofloxacin at 1x, 2x and 4x MIC on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 were studied using a spectrophotometric method and the classic method of viable counts on agar as a reference. Monitoring of the growth kinetics was carried out by viability counting on the plate every hour and by...

The Limulus lysate assay was used to measure the endotoxin content in stream water and was found to reflect the degree of bacterial contamination as measured by coliform, enteric, gram-negative, and heterotrophic bacteria. The firm-clot method was found to be a less sensitive and reproducible technique for the detection of endotoxin than was the spectrophotometric modification of the Limulus ly...

With this fluorometric method for measuring indocyanine green (ICG) in biological fluids, the limit of detection is an order of magnitude lower than for the traditional spectrophotometric procedure. The excitation and emission maxima are 780 and 810 nm, respectively. Agreement was excellent (r = 0.998) between direct results by this method and those by a liquid-chromatographic procedure with sp...

We describe an enzymatic fluorometric method for determining glucose concentrations in blood samples by analysis on a semi-solid surface (silicone-rubber pads). The method is an adaptation of existing spectrophotometric procedures based on the sequential coupling reactions with glucose-6-phosphate dehydrogenase and NADP+. The rate of appearance of NADPH fluorescence at 460 nm (excitation wavele...

Simple and accurate spectrophotometric and HPLC method was developed for determination of Fexofenadine in tablets dosage form. The spectrophotometric method was developed by dissolving tablets in 1:1 Methanol to make solution of 10ppm giving absorbance at 220nm. The experimental conditions were optimized and Beers law was obeyed over the applicable concentration range. The application of HPLC p...

The m-aminophenylarsonic acid (m-APAA) was immobilized onto the silica gel surface with covalently grafted quaternary ammonium groups via ion exchange. The diazotization of ion-bonded m-APAA resulted in a new solid-phase spectrophotometric reagent for detection of 1-naphtol in environmental water samples. The procedure of solid-phase spectrophotometric analysis is characterized by 20 μg L(-1) l...

A simple and rapid method has been developed for the quantification of Domperidone (DMP) and Paracetamol (PAR) through FT-IR in combined dosage form. The method involves the measurements of peaks of carbonyl group (C=O) at 1656 cm (PAR) and 1717 cm (DMP). An UV-Spectrophotometric method was also described for the simultaneous determination of DMP and PAR. The analytical results obtained with FT...