A method that gives high recovery of deoxyribonucleic acid (DNA) from crude bacterial lysates using ethidium bromide-cesium chloride density gradient centrifugation is presented. After Pronase digestion and shearing of the lysate, essentially 100% recovery of chromosomal DNA and a reproducible recovery of covalently closed circular (CCC) plasmid DNA is obtained for a specific plasmid in a given...

Transgenic tobacco roots offer a potential alternative to leaves for monoclonal antibody (MAb) production. A possible method for extraction of MAbs from roots is by homogenization, breaking the roots into fragments to release the antibody. This process was assessed by shearing 10 mm root sections ("roots") in a 24 mL ultra-scale down shearing device, including an impeller with serrated blade ed...

Glaucomatous optic neuropathy may be in part due to an altered biomechanical environment within the optic nerve head (ONH) produced by an elevated intraocular pressure (IOP). Previous work has characterized the magnitude of the IOP-induced deformation of ONH tissues but has not focused specifically on the mode of deformation (strain), i.e. whether the ONH tissues and cells are stretched, compre...

Asymptotic regimes of geophysical dynamics are described for di erent Burger number limits. Rotating Boussinesq equations are analyzed in the asymptotic limit of strong strati cation in the Burger number of order one situation as well as in the asymptotic regime of strong strati cation and weak rotation. It is shown that in both regimes horizontally averaged buoyancy variable is an adiabatic in...

Delamination is a critical form of failure in composite laminates which is partitioned into a combination of three pure modes namely; opening, shearing and tearing (modes I, II and III respectively). Each fracture mode contributes to the total energy required for delamination growth, due to a combination of the different damage mechanisms that occur under each mode 1-2 . To formulate reliable m...

A dilute gas of particles with short range interactions is considered in a shearing stationary state. A Gaussian thermostat keeps the total kinetic energy constant. For infinitely many particles it is shown that the thermostat becomes a friction force with constant friction coefficient. For finite number of particles N, the fluctuations around this constant are of order 1/squareroot[N], and dis...

The average separation of two-dimensional islands in Cu(100) submonolayer homoepitaxy as a function of the deposition flux at 213 K has been studied using spot profile analysis low-energy electron diffraction. As the flux decreases, a large change in the apparent critical island size, from one to a value between seven and 12 atoms, is obtained even though the temperature is held constant. This ...