نتایج جستجو برای: specific primer combinations were designed with expected amplicon sizes of 500 bp from m fijiensis

تعداد نتایج: 22684442  

A Razei, H Aghamollaei , R Sorouri , SL Mousavi ,

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...

Journal: :Experimental parasitology 2009
Mohammad A Oshaghi Naseh Maleki Ravasan Mallorie Hide Ezat-Aldin Javadian Yavar Rassi Mohammad M Sedaghat Mehdi Mohebali Homa Hajjaran

Discrimination of Leishmania infantum and L. donovani, the members of the L. (L.) donovani complex, is important for diagnosis and epidemiological studies of visceral leishmaniasis (VL). We have developed two molecular tools including a restriction fragment length polymorphisms of amplified DNA (PCR-RFLP) and a PCR that are capable to discriminate L. donovani from L. infantum. Typing of the com...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده دامپزشکی 1389

چکیده: بررسی مقایسه ای گونه های مختلف مالاسزیا در پوست و گوش گوساله های نوزاد با مادرانشان با استفاده از روشهای مورفولوژیکی، بیوشیمیایی و فیزیولوژیکی مخمرهای لیپوفیلیک جنس مالاسزیا جزء فلور نرمال جلدی انسان و حیوانات می باشند که تحت شرایطی بیماریزا می شوند. از آنجایی که نه تنها گونه غیر وابسته به چربی بلکه سایر گونه های مالاسزیا از حیوانات مختلف جداسازی می شود لذا بررسی اپیدمیولوژی و انتشار ج...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علامه طباطبایی - دانشکده علوم اجتماعی 1393

the present study is paid to the evaluation of the welfare program of the unemployment insurance in iran. the main purpose which was the main reason for performing this thesis, was the unemployment insurance plan’s challenges in iran such as financial problems of this plan, prolongation of the credit receipt for some insured people, unemployment slow exiting from the unemployment insurance fund...

Journal: :journal of cell and molecular research 0
seyed ali mortazavi ahmad reza bahrami balal sadeghi maryam moghaddam matin

in present study, a sybr green based real time pcr assay was developed for specific detection and quantification of bacillus subtilis in dough used for bread making. new primer pairs were designed to amplify a 212 base pair fragment of the apre gene. specificity of these primer pairs was confirmed with conventional and real time pcr methods. standard curves constructed using the threshold cycle...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده زبانهای خارجی 1389

previous studies have aimed at testing a hypothesis but the present study is data-first research in which researcher tries to form a theory from data rather than the use of data to test a hypothesis. this study aims at exploring teachers perceptions of the strengths and weaknesses of the current syllabus for college preparatory course. eight experienced teachers were selected to collect data du...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده زیست شناسی 1388

126 abstract: in this research we studied the effects of cyclic hydration and dehydration of cysts of artemia urmiana and artemia parthenogenetica on the hatching percentage, survival and growth. the experiment was carried out in 3 treatments (1-3 hydration/dehydration cycles) with 3 replicates for each treatment. later effects of cold preservation at -20°c during three time periods were ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان - دانشکده علوم پایه 1391

abstract the balvard metamorphic assemblage (bma) crops out at the northeastern margin of the southeastern part of the sanandaj-sirjan zone (ssz) in contacting to the dehshir-baft ophiolitic mélange. this location prepared a special situation to resolve some of the complicated tectonic problems of this part of iran. petrology, micro-tectonics and satellite image investigation were carried out ...

اسفندیاری, عزت اله, سفالیان, امید, شکرپور, مجید , صارمی راد, بابک , پورمحمد, علیرضا ,

Genetic diversity of 45 wheat varietions were studied using AFLP markers. From 11 primer combinations of PstI and Mse1for obtaining AFLP polymorphism, six primer combinations (P-CGA/M-CTA, P-ATG/M-CTG, P-ATG/M-CAT, P-AGT/M-CTA, P-AGT/M-CAG and P-ATG/M-CAG) produced polymorphic bands. Totally, 328 bands be produced that 207 bands (63.10%) were polymorphic, which showing high polymorphism among g...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تهران - دانشکده علوم 1370

تهیه ترکیب فسفرتری کلرید بااستفاده از واکنش فسفر سفید(زرد) و گاز کلر در فاز محلول و درمقیاس آزمایشگاهی موردمطالعه قرارگرفت .پس از انتخاب مناسبتر روش و انتخاب بهترین شرایط کوشش درجهت طراحی واحد نیمه صنعتی انجام شد و توانستیم دراین راستا به تولید 10 کیلوگرم درساعت باخلوص 99/5 درصد برسیم . abstract the preparation of phosphorous trichloride by the reaction of white phosphorous and chlorine gas in ...

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