Cycle-linear hybrid automata (CLHAs), a new model of excitable cells that efficiently and accurately captures action-potential morphology and other typical excitable-cell characteristics such as refractoriness and restitution, is introduced. Hybrid automata combine discrete transition graphs with continuous dynamics and emerge in a natural way during the (piecewise) approximation process of any...

Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an a...

Host cell proteins (HCPs) must be adequately removed from recombinant therapeutics by downstream processing to ensure patient safety, product quality, and regulatory compliance. HCP process clearance is typically monitored by enzyme-linked immunosorbent assay (ELISA) using a polyclonal reagent. Recently, mass spectrometry (MS) has been used to identify specific HCP process impurities and monito...

During the last ten years the extraction of specific antibodies (ab) from the yolk of eggs of immunised chickens is more and more accepted as an useful alternative to the immunisation of mammals. The subject of this work is the immunisation of chickens with human IgG and the extraction of specific anti human IgG ab from egg yolk in order to obtain monospecific Coombs reagent. 12 Leghorn hens (2...

organophosphorus compounds are known to inhibit the esteratic site of acetylcholinesterase by phosphorylation. the phosphorylated esteratic site of acetylcholinesterase undergoes hydrolytic regeneration at a slow or negligible rate. nucleophilic agents such as hydroxytamine, hydroxamic acids, and oximes reactivate the enzyme more erapidfy than does spontaneous hydrolysis. the red cell cholinest...

The coagglutination (CoA) method for the identification of Neisseria gonorrhoeae colonies grown on selective culture media was used on 116 strains in a routine venereological laboratory together with the direct immunofluorescence (IF) test and the sugar fermentation reaction. Correlation of results between the CoA method and the direct IF test and between the CoA test and the sugar fermentation...

this study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (sbp) in cirrhotic patients. forty six children with ascites hospitalized between 2009 and 2010 in children medical center were enrolled in this study. reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

The pharyngeal aspirates collected from 400 babies at the time of delivery were examined for the presence of bacteria, especially group B streptococci. Aspirates from 79 babies were found to contain viable bacteria, including 4 with group B streptococci; one of these 4 babies developed streptococcal meningitis within 24 hours. The group B streptococci were seen on a Gram-stained film of the asp...