To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for cal...

to explore the potential for in vitro rapid regeneration of fagonia indica burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on ms medium which supplemented with 1.0-5.0 µm 2,4-d. callus was initiated from cotyledon explant on ms medium supplemented in all concentrations of 2,4-d investigated. 2,4-d was more effective than iaa and naa for cal...

Background: Somatic cell nuclear transfer, commonly known as cloning is a powerful tool for the production of identical animals for productive, biomedical or conservation purposes. Today several species of mammals has been cloned using this technology. Nevertheless, the efficiency of the process is still low in terms of newborn animals. The causes for such inefficiencies are mainly the lack of ...

We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable a...

The aim of this study was to evaluate different mating strategies among endogamic strains to create F1 populations of mice, minimising the effect of inbreeding depression on somatic development and embryo yield. Females from the strains Swiss, CBA and C57Bl/6 were divided in nine experimental mate arrangements. The total numbers of pups born alive per dam and somatic development, estimated by w...