نتایج جستجو برای: somaclonal variation

تعداد نتایج: 296663  

2015
R. Karim F. Ahmed Krishna Roy M. Hossain

Strawberry is a valuable, nutritious, and economically important fruit all over the world including Bangladesh. Therefore, there is a demand to develop a suitable variety of strawberry. For this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto MS medium supplemented with different concentrations and combinations of 2,4-D, NAA and BA for callus induction. The mo...

2013
Hume Stroud Bo Ding Stacey A Simon Suhua Feng Maria Bellizzi Matteo Pellegrini Guo-Liang Wang Blake C Meyers Steven E Jacobsen

Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps of DNA methylation in several regenerated rice lines. We found that all tested regenerated plants had significant losses of methylation compared to non-regenerated plants. Loss of methylatio...

2013
Taiji Kawakatsu Yoshihiro Kawahara Takeshi Itoh Fumio Takaiwa

Genetic modification (GM) by Agrobacterium-mediated transformation is a robust and widely employed method to confer new traits to crops. In this process, a transfer DNA is delivered into the host genome, but it is still unclear how the host genome is altered by this event at single-base resolution. To decipher genomic discrepancy between GM crops and their host, we conducted whole-genome sequen...

Journal: :The Plant journal : for cell and molecular biology 2016
Fu-Jin Wei Lin-Yun Kuang Hui-Min Oung Sin-Yuan Cheng Hshin-Ping Wu Lin-Tzu Huang Yi-Tzu Tseng Wan-Yi Chiou Vicki Hsieh-Feng Cheng-Han Chung Su-May Yu Lan-Ying Lee Stanton B Gelvin Yue-Ie C Hsing

Rice (Oryza sativa) is one of the world's most important crops. Rice researchers make extensive use of insertional mutants for the study of gene function. Approximately half a million flanking sequence tags from rice insertional mutant libraries are publicly available. However, the relationship between genotype and phenotype is very weak. Transgenic plant assays have been used frequently for co...

Journal: :Journal of experimental botany 2011
Célia Miguel Liliana Marum

Epigenetic mechanisms are highly dynamic events that modulate gene expression. As more accurate and powerful tools for epigenetic analysis become available for application in a broader range of plant species, analysis of the epigenetic landscape of plant cell cultures may turn out to be crucial for understanding variant phenotypes. In vitro plant cell and tissue culture methodologies are import...

2010
R. QU A. H. BRUNEAU O. A. Rognli

St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is the least cold-hardy turfgrass species. Development of freezing-tolerant St. Augustinegrass cultivars would greatly benefit home owners in many southern states of the US. Towards this breeding goal, 7800 plants regenerated through tissue culture and 36 germplasm accessions were screened for improved freezing tolerance. Among the con...

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