BACKGROUND In the past few years, both automated and manual high-throughput protein expression and purification has become an accessible means to rapidly screen and produce soluble proteins for structural and functional studies. However, many of the commercial vectors encoding different solubility tags require different cloning and purification steps for each vector, considerably slowing down e...

The expense, length of time and number of animals required for routine toxicity testing have provided the incentive for finding alternative techniques which are faster, less expensive and equally valid. The purpose of this work was to examine the value of a simple in vitro test (hemolysis) as a correlate of the neurotoxicity produced by commonly used industrial organic solvents. Incubation of r...

10-Hydroxycamptothecin (10-HCPT) is a promising anticancer drug with a wide spectrum of antitumor activities. Due to its poor solubility, the carboxylate form that shows high water solubility but minimal anticancer activity and pharmacokinetic defects is used in the marketed 10-HCPT injections, resulting in its limited clinical application. To develop a simple, safe, and highly effective drug d...

It is known that many potent, often aromatic drugs are water insoluble, which has hampered their use for disease treatment. In this work, we functionalized nanographene oxide (NGO), a novel graphitic material, with branched polyethylene glycol (PEG) to obtain a biocompatible NGO-PEG conjugate stable in various biological solutions, and used them for attaching hydrophobic aromatic molecules incl...

Telmisartan (TLM) is an angiotensin II receptor antagonist used in the treatment of hypertension. According to BCS (biopharmaceutical classification system) Telmisartan belongs to class II drug, and it is practically insoluble in water and it shows low dissolution profile and poor absorption. The present study is to improve the solubility of Telmisartan by solid dispersion techniques using vari...

Equations have been derived and computations carried out for the demineralization rates of tooth enamel employing the “Fluorapatite” model. The model assumes that when hydroxyapatite is exposed to a low fluoride concentration, a layer of fluorapatite is formed around the apatite crystal and this fluorapatite is assumed to be the thermodynamic governing phase during dissolution. The demineralixa...

The approximate solubility products of the calcium salts of ten unconjugated bile acids and several taurine conjugated bile acids were determined. The formation of micelles, gels, and/or precipitates in relation to Ca2+,Na+, and bile salt concentration was summarized by "phase maps." Because the ratio of Ca2+ to bile salt in the precipitates was ca. 1:2, and the activity of Ca2+ but not that of...

We propose a new electrochemical method for the measurement of solubility product oxide metal impurity, in liquid solvent that is less easily oxidized than impurity. When known amount oxygen continuously added into by coulometric titration, dissolved impurity reacts with oxygen, forming oxide. By measuring concentration during can be derived. From this product, using thermochemical data chemica...