Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity transporter. In this study, analysis of an uptake- and sensing-decoupled mutan...

A small microcosm, based on optimized in vitro transformation conditions, was used to study the ecological factors affecting the transformation of Acinetobacter calcoaceticus BD413 in soil. The transforming DNA used was A. calcoaceticus homologous chromosomal DNA with an inserted gene cassette containing a kanamycin resistance gene, nptII. The effects of soil type (silt loam or loamy sand), bac...

Proper N fertilizer and irrigation management can reduce nitrate leaching while maintaining crop yield, which is critical to enhance the sustainability of vegetable production on soils with poor water and nutrient-holding capacities. This study evaluated different methods to measure nitrate leaching in mulched drip-irrigated zucchini, pepper, and tomato production systems. Fertigation rates wer...

Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.

Streptomyces griseorubens JSD-1, isolated from compost-treated soil, is able to utilize lignocellulose and nitrate as its sole carbon and nitrogen source for growth. Here, we announce the draft genome map of this actinomycete. The genes participating in lignocellulose and nitrate metabolism were picked out and identified.

nitrate concentration of the soil, at the start of growing season, is high due to minerali-zation of soil organic n during the fall and the addition of n fertilizers. it may be useful to exploit this n form as much as possible. therefore, the nitrate uptake, assimilation and dry matter production of several wheat cultivars were examined in a series of experiments using a hydroponic culture syst...

The capacity of mycorrhizal and non-mycorrhizal root systems to reduce nitrate (NO₃⁻) and ammonium (NH₄⁺) loss from soils via leaching was investigated in a microcosm-based study. A mycorrhiza defective tomato mutant and its mycorrhizal wildtype progenitor were used in this experiment in order to avoid the indirect effects of establishing non-mycorrhizal control treatments on soil nitrogen cycl...

The hydrological and biogeochemical monitoring of catchments has become a common approach for studying the effect of the evolution of agricultural practices on water resources. In numerous studies, the catchment is used as a “mega-lysimeter” to calculate annual input-output budgets. However, the literature reflects two opposite interpretations of the trends of nitrate concentration in streamwat...