A fluorescence probe study on micellization of the binary surfactant system formed by n-octyl-d-thioglucopyranoside (OTG) and sodium dodecyl sulphate (SDS) in 0.1 M NaCl aqueous solutions has been carried out. The CMC experimental data, as obtained by the pyrene 1:3 ratio method, have been analyzed in the context of the pseudophase separation model. The interaction parameter (β12) determined by...

Sodium dodecyl sulphate (SDS) was used to weaken both the electrostatic and the hydrophobic interactions during collagen fibrillogenesis in vitro. The rate and extent of fibril formation as well as fibril morphology were affected by SDS concentration. Both the formation of large fibrils at 0.3 mM-SDS and the complete cessation of fibril formation at 0.5 mM-SDS were considered to be the result o...

Intraperitoneal administration of sodium oestrone [(35)S]sulphate to male and female free-ranging guinea pigs is followed by excretion of most of the radioactivity mainly as inorganic [(35)S]sulphate in the urine within 72h. The remainder of the radioactivity in the urine was found in oestrone [(35)S]sulphate, two unidentified metabolites (A and B) and traces of oestradiol-17beta 3-[(35)S]sulph...

A Study on the binding of sodium dodecyl sulphate (SDS) and sodium octyl sulphate to gliadin was made by employing dialysis equilibrium method. The binding data reveals that the rising molar concentration of the surfactants correspond to the changes in the binding process. In the lower concentration range (region A) of the surfactants, the plot VM Vs log Cf represented linear behaviour. This re...

Some N-alkyl pia-hydroxy styryl pyridinium halides, the alkyl moieties being methyl, hexyl and decyl, have been synthesised and the acid dissociation constants of these compounds in aqueous and surfactant solutions have been determined using spectrophotometric method . The alkyl chain does not have any substantial effect on the pKa values in aqueous and cationic surfactant (cetyl trimethylammon...

A major periodate--Schiff-positive component from milk-fat-globule membrane of human breast milk has been purified by selectively extracting the membrane glycoproteins, followed by lectin affinity chromatography and gel filtration on Sephadex G-200 in the presence of protein-dissociating agents. The purified glycoprotein, termed epithelial membrane glycoprotein (EMGP-70), has an estimated mol.w...

(2002) Introducing Wilson disease mutations into the zinc-transporting P-type ATPase of Escherichia coli. The mutation P634L in the " hinge " motif (GDGXNDXP) perturbs the formation of the E 2-P state. Eur. The nucleotide-binding domain of the Zn 2+-transporting P-type ATPase from Escherichia coli carries a glycine motif that may be involved in binding of ATP. Biochem. J. 377: 95-105 In additio...

A glutathione S-transferase (GST) was purified from an arsenic-resistant Chinese hamster ovary cell line, SA7. The SA7 GST was shown to catalyse the conjugation of glutathione and ethacrynic acid, a specific substrate for Pi class GST. Its N-terminal amino-acid sequence has 80% identical residues to that of rat GST P and human GST pi. Thus, the GST purified from SA7 cells belongs to the Pi fami...

Kinetic parameters (Km and kcat.) of the two major forms (A and B) and a minor form (C) of human liver N-acetylglucosamine-6-sulphate sulphatase [Freeman, Clements & Hopwood (1987) Biochem. J. 246, 347-354] were determined with a variety of substrates matching structural aspects of the physiological substrates in vivo, namely heparin, heparan sulphate and keratan sulphate. Enzyme activity is hi...