A nucleotide pyrophosphatase (EC 3.6.1.9) has been purified from extracts of the hybrid yeast, Saccharomyces fragilis X Saccharomyces dobzhanskii, to an extent where it appears homogeneous by ultracentrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Multiple protein components seen on polyacrylamide and starch gel electrophoresis also exhibit enzyme activity. Ultracentr...

We have identified a yeast protein that resembles actins from other eucaryotes in its tight binding to pancreatic deoxyribonuclease I, its copolymerizaton with purified muscle actin, its one-dimensional peptide map, and its apparent polymerization into 7-nm filaments. The yeast actin-like protein yielded a single spot on two-dimensional polyacrylamide gel electrophoresis, suggesting that a sing...

A hemolysin produced by a strain of Aeromonas hydrophila isolated from a patient with diarrhea was purified by acid precipitation and quarternary aminoethyl-Sephadex chromatography. The molecular weight of the hemolysin was estimated at 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and at 48,000 by Sephadex G-100 gel filtration. In polyacrylamide gel electrophoresis at pH ...

Mouse myeloid leukemic M1 cells are induced to differentiate by various differentiation inducers. Activity for inhibition of induction of differentiation of M1 cells (I-factor activity) was detected in conditioned medium of variant M1 cell clones that were resistant to differentiation inducers, and this I-factor activity was shown to be closely associated with resistance of the cells to differe...

A phospholipase A1 bound tightly to the membranes of Mycobacterium phlei cells was purified approximately 500fold to near homogeneity by extraction with Triton X-100, delipidation with organic solvents, solubilization with sodium dodecyl sulfate, column chromatographies on Sephadex G-200 in the presence of sodium dodecyl sulfate and on DEAE-cellulose in the presence of BRIJ 58, and sodium dodec...

Synaptosomal plasma membranes were isolated from whole rat brain by a method derived from several existing ones. The procedure involves the sequential centrifugation of a crude mitochondrial fraction through a discontinuous sucrose gradient and a continuous cesium chloride gradient. The material is then subjected to osmotic lysis and centrifuged through an additional discontinuous sucrose gradi...

A phospholipase A1 bound tightly to the membranes of Mycobacterium phlei cells was purified approximately 500fold to near homogeneity by extraction with Triton X-100, delipidation with organic solvents, solubilization with sodium dodecyl sulfate, column chromatographies on Sephadex G-200 in the presence of sodium dodecyl sulfate and on DEAE-cellulose in the presence of BRIJ 58, and sodium dodec...

Antibody directed against c factor from vegetative Bacillus subtilis was prepared by immunizing a rabbit with denatured cr polypeptide isolated by electrophoresis of partially purified DNA-dependent RNA polymerase on a sodium dodecyl sulfate-polyacrylamide slab gel. Antiserum to (r reacted specifically with native c polypeptide but not with core RNA polymerase as judged by complement fixation a...

A protein capable of activating 6-aminolevulinic acid synthetase has been purified from rat liver mitochondria. By treatment of the mitochondrial inner membrane-matrix fraction with Lubrol a soluble and active preparation of 6-aminolevulinic acid synthetase has been obtained. Ammonium sulfate fractionation yielded a 33 to 40% saturated fraction containing catalytic 6-aminolewlinic acid syntheta...

Ciliary neurotrophic factor (CNTF) has been purified 35,000-fold to homogeneity from rabbit sciatic nerves using its ability to promote the survival of chick embryo ciliary ganglion neurons as the bioassay. The purification involved a combination of acid treatment, ammonium sulfate fractionation, hydrophobic interaction chromatography, chromatofocusing, preparative sodium dodecyl sulfate-polyac...