Identification of polymorphism is the key for genetic mapping, diagnosis and marker-assisted selection. Restriction fragment-length polymorphisms (RFLPs) and short tandem repeats (STRs), also known as microsatellites, are commonly used genetic markers. Until discovery of STRs, RFLPs were used for genetic mapping, but bi-allelic polymorphism of RFLP limited its use as a marker. Although STRs are...

staphylococcus aureus carrying pvl gene remain major health problem associated with highly virulent infections. characterization of such gene is important to know the impact and the functional significance of nucleotide variations. pcr and standard sequencing were performed for twelve sudanese strains from different sources. protein structures prediction, modeling and physiochemical analysis we...

Platelet-dependent thromboembolism is an underlying mechanism in the pathogenesis of stroke. 5-HT2A receptor gene is expressed in human platelet, coronary artery (blood vessels) and brain. A polymorphism T102C at the 5-HT2A receptor gene was found that may possibly affect the 5-HT2A receptor function. As there is no existing data on T102C variant of 5-HT2A receptor gene in pediatric stroke, we ...

stem cell factor (scf) is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. in the present study a comparative analysis on nucleotide sequences of scf was performed in humanoids using bioinformatics tools including ncbi-blast, mega6, and jbrowse. our analysis of nucleotide sequences to find closely evolved organisms with high similarity by ncb...

Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to c...

Single nucleotide polymorphism (SNP) detection technologies are used to scan for new polymorphisms and to determine the allele(s) of a known polymorphism in target sequences. SNP detection technologies have evolved from labor intensive, time consuming, and expensive processes to some of the most highly automated, efficient, and relatively inexpensive methods. Driven by the Human Genome Project,...

The present study was carried out to investigate the association of C/T single nucleotide polymorphism(SNP)in exon 5 of stearoyl-CoA desaturase 1 (SCD1) gene and A/C SNP in the 3' untranslated region of oxidized low density lipoprotein receptor 1 (OLR1)gene with milk production traits in Iranian Holstein dairy Cattle. The blood samples of 153 (for OLR1) and 308 (for SCD1) dairy cattle from thre...

UNLABELLED SNPNB is a user-friendly and platform-independent application for analyzing Single Nucleotide Polymorphism NeighBoring sequence context and nucleotide bias patterns, and subsequently evaluating the effective SNP size for the bias patterns observed from the whole data. It was implemented by Java and Perl. SNPNB can efficiently handle genome-wide or chromosome-wide SNP data analysis in...

mastitis is one of the most serious and costly diseases affecting dairy cattle production. in the present study, effects of a lactoferrin gene polymorphism (intron 6) on milk somatic cell count (scc) and subclinical mastitis was investigated in 121 holstein dairy cattle. two alleles of a and b and two genotypes of aa and ab were found in an ecori recognized single nucleotide polymorphism in int...