Succinic acid is identified to have a great economical potential in a biobased economy. To improve the production in Escherichia coli metabolic engineering should be carried out. For new mutant strain design is important to in silico simulate and analyze the metabolic changes, network of a cell under different environmental and/or genetic perturbations. To investigate the genetic and environmen...

The pathway of aromatic amino acid biosynthesis was initiated by the enzyme DAHP synthase. In this branched biosynthetic pathway phenylalanine, tyrosine and tryptophan are produced. This enzyme was repressed and inhibited by three said end product of aromatic amino acids. The importance of this enzyme is microbial fermentation of aromatic amino acid production. The present study envisaged the i...

There are a number of in silico programs that use algorithms and external web sources to predict the effect of single nucleotide polymorphisms (SNPs). While many of these programs have been shown to predict accurately the effect of SNPs in functional areas of the gene, such as 5' upstream or coding regions, empiric research may be warranted to confirm the functional consequences of SNPs that ar...

In the present study we have tried to explore the possibilities of applying in silico approaches for understanding the concept of relatedness between closely related species of actinomycetes. Various biochemical tests suggested the presence of SD9 as most promising isolate with potential antibiotic activity and in silico analysis by Neighbor-Joining method using MEGA software suggested a closel...

New peptides with potential antimicrobial activity, encrypted in milk protein sequences, were searched for with the use of bioinformatic tools. The major milk proteins were hydrolyzed in silico by 28 enzymes. The obtained peptides were characterized by the following parameters: molecular weight, isoelectric point, composition and number of amino acid residues, net charge at pH 7.0, aliphatic in...

OBJECTIVE The aim of this study was to investigate the role of APE1 Asp148Glu polymorphism in breast cancer progression in Saudi population. METHODS We examined the genetic variations (rs1130409) in the DNA base excision repair gene APE1 at codon 148 (Asp148Glu) and its association with breast cancer risk using genotypic assays and in silico structural as well as functional predictions. In si...