We provide here evidence that supports the occurrence of a biologically dormant form of selectin ligand carbohydrate, the sialyl 6-sulfo Lewis X containing modified sialic acid, in human leukocytes. The modification of sialic acid involves first de-N-acetylation of sialic acid moiety through ubiquitous de-N-acetylation/re-N-acetylation cycle, followed by the dehydrative cyclization of de-N-acet...

OBJECTIVE To determine whether serum sialic acid concentration may be used to predict short and long term cardiovascular mortality. DESIGN Prospective study on all men and women who had their serum sialic acid concentration measured as part of a general health survey in 1964 or in 1965. All were followed up for an average of 20.5 years. SETTING Geographical part of the county of Värmland, S...

The presence of sialic acid on cell membranes was found to be necessary for the outward flow of proteins from L1210 leu kemia cells. The removal of membrane-bound sialic acid inhib ited the release of proteins without any effect on cell lysis, or on the release of nucleotides or sugars. The requirement for sialic acid in promoting protein secretion seemed relatively specific for certain protein...

The presence of sialic acid on cell membranes was found to be necessary for the outward flow of proteins from L1210 leu kemia cells. The removal of membrane-bound sialic acid inhib ited the release of proteins without any effect on cell lysis, or on the release of nucleotides or sugars. The requirement for sialic acid in promoting protein secretion seemed relatively specific for certain protein...

Sialic acids decorate the surfaces of most mammalian cells and are used by many viruses as attachment receptors. In contrast to other mammals, humans cannot synthesize a version of sialic acid known as N-glycolyl neuraminic acid. This difference is exploited by some viruses to establish tropism. Here we compare recently determined structures of closely related animal and human polyomaviruses an...

The Siglecs are a recently discovered family of sialic acid-binding lectins of the immunoglobulin (Ig) superfamily. We report a molecule showing homology to the six first reported Siglecs, with the closest relationship to Siglec-3(CD33), Siglec-5, and Siglec-6(OBBP-1). The extracellular portion has two Ig-like domains, with the amino-terminal V-set Ig domain including amino acid residues known ...

Three C terminal His6-tagged recombinant microbial CMP-sialic acid synthetases [EC 2.7.7.43] cloned from Neisseria meningitidis group B, Streptococcus agalactiae serotype V, and Escherichia coli K1, respectively, were evaluated for their ability in the synthesis of CMP-sialic acid derivatives in a one-pot two-enzyme system. In this system, N-acetylmannosamine or mannose analogs were condensed w...

To evaluate the possibility that the carbohydrate composition of fibrinogen may be altered in the dysfibrinogenemia associated with liver disease, we studied the sialic acid content of purified fibrinogen from 12 patients with liver disease and its relationship to the prolongation of the thrombin time. Purified fibrinogen showed that Aalpha-, Bbeta-, and gamma-chains when reduced and analyzed b...

A plasmonic bioassay for the specific detection of human influenza virus has been developed based on gold nanoparticles functionalised with a designed and synthesised thiolated trivalent α2,6-thio-linked sialic acid derivative. The glyconanoparticles consist of the thiolated trivalent α2,6-thio-linked sialic acid derivative and a thiolated polyethylene glycol (PEG) derivative self-assembled ont...

changed, whereas hexosamine values were gradually increased and seemed to be correlated to total cell count, but not to the count of adhered cells on the glass wall. On the other hand, hexosamine and sialic acid contents of both the cells adhering to the glass wall were determined after washing with saline solution. The results are given in Table I. No remarkable difference in hexosamine and si...