Cotyledonary node explants excised from 21 day old seedlings of T. arjuna produced multiple shoots when cultured on full strength MS or modified MS (1/2 strength major salts and Fe-EDTA) medium supplemented with different concentrations (0.1-1.0 mg/l) of BAP. Maximum 8.9 shoots/explant could be recorded after 30 days of inoculation on modified MS medium supplemented with BAP (0.5 mg/l). A proli...

Pterocarpus santalinus belongs to fabaceae family and is one of the endemic and endangered medicinal plant species of seshachalam hills, Tirumala, Andhra Pradesh state, India. In the present study we have successfully demonstrated the in vitro regenartion of this plant through shoot bud culture. The seeds germinated sequentially on plastic trays and transferred polythene bags for 21 days served...

Objective: Here, we established the protocol for plant regeneration of Psoralea corylifolia via In-vitro micro propagation. Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine (BAP), Kinetin (Kn), 1-Naphthaleneacetic acid (NAA) and B5 vitam...

An improved organogenesis protocol for multiplication of sweet orange cv. Mosambi has been standardized by the use different PGRs, basal media and ethylene adsorbents. Incorporation cytokinins, BAP (8.8 µM) kinetin (6.97 resulted in best shoot with highest response (81.40%), no. micro-shoots/explant (2.06), mean micro-shoot length (1.30 cm) leaves/micro-shoot (3.55) owing to complementary effec...

More than 50% out of 129 Agave species are endemic to Mexico. Among them, peacockii is among the list threatened that require special protection. In this work, we aimed at developing new supplementary strategies achieve micropropagation and perform cryopreservation in vitro grown shoot-tips A. peacockii. For multiplication, addition two cytokinins, 6-benzylaminopurine (26.6 μM) kinetin (27.84 M...

Bambusa balcooa Roxb. was in vitro propagated by optimizing protocol using nodal segment from secondary branches with 100% success MS liquid media containing 100 mg/l Myo-inositol, 3% sucrose supplement 4.4 - 26.64 ?M BAP for shoot multiplication, and 2.69 32.26 NAA root induction. The highest multiplication (14.53 ± 0.33 folds), length (5.9 0.6 cm), number per explants (4.0 0.24), rooting (89....

Stevia plant contains steviol glycosides, which are estimated to be 300 times sweeter than sucrose. However, conventional ( in vivo ) propagation of rebaudiana Indonesia was not effective due poor results. Therefore, an alternative method propagate stevia plants is needed. One them by using vitro method. Multiplication with a large quantity biomass relatively short period can conducted TIS RITA...

In vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. In the present investigation, the objectives were to optimize the micropropagation of Rose hybrid ‘Black Baccarat’ cultivar. In proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (MS, ...

Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask r...

Atriplex taltalensis is an endangered shrub endemic to northern Chile. Sparse populations of this species can be found in areas with extreme edaphoclimatic conditions the Atacama desert, and its seeds have a poor germination rate. Consequently, mass-cultivating it challenging. In study, vegetative propagation mechanisms A. were evaluated using vitro culture aid their conservation. shoot explant...