Multiple shoots were achieved through axillary bud explant of Spilanthes acmella on MS medium supplemented with 0.5mg/l BA. In vitro developed axillary buds and tiny shoots isolated from the multiple shoots were encapsulated in different strength of sodium alginate. The present work explains the role of components of culture medium on morphogenic response of encapsulated shoot tips to various t...

Rye is second only to wheat among grains most widely used in the making of bread and is also a very important gene resource for breeding and improvement of wheat and other cereal crops owing to tolerance to abiotic stress factors such as low temperatures, drought and poor soil conditions. However, application of biotechnologies has been limited in rye breeding since it is one of the most recalc...

Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

Development of new techniques for in vitro culture is necessary to optimize and reduce the production costs. The aim of this work was to introduce the bioreactor BIB (Bioreactor of Immersion by Bubbles) and compare it with conventional culture and bioreactor RITA (Recipient with Automatized Temporary Immersion) for In vitro growth of M. alternifolia plantlets. Plantlets with 1.0 cm height from ...

An in vitro propagation system was developed to obtain shoot and root cultures from the Andean spice Sanicula graveolens (Apiaceae). Propagation of shoots, roots and plantlets was achieved by the temporary immersion system. The free radical scavenging effect of the methanol/water (7:3 v/v) extracts was determined by the discoloration of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Total ph...

Tissue culture provides a useful system to investigate how plant hormones are involved in this process. Auxin and cytokinin are widely used in plant regeneration. Gibberellin is also an important plant hormone in regulating plant growth and development. It is interesting to know the effects of gibberellin and its signalling pathway on plant regeneration. In this report Arabidopsis thaliana land...