Calcitriol, acting through vitamin D receptors (VDR) in the parathyroid, suppresses parathyroid hormone synthesis and cell proliferation. In secondary hyperparathyroidism (SH), VDR content is reduced as hyperplasia becomes more severe, limiting the efficacy of calcitriol. In a rat model of SH, activation of the EGF receptor (EGFR) by TGF-alpha is required for the development of parathyroid hype...

Understanding how neural cells handle proteostasis stress in the endoplasmic reticulum (ER) is important to decipher the mechanisms that underlie the cell death associated with neurodegenerative diseases and to design appropriate therapeutic tools. Here we have compared the sensitivity of a human neuroblastoma cell line (SH-SY5H) to the ER stress caused by an inhibitor of protein glycosylation ...

In SH-SY5Y, a human neuroblastoma cell line, Aroclor 1254 (A1254), induced a dose-dependent (10-50 microg/ml) intracellular calcium concentration ([Ca2+]i) increase. Two rather specific sodium-calcium (Na+-Ca2+) exchanger (NCX) inhibitors, bepridil (10 microM) and KB-R7943 [2-[2-[4-(4-nitrobenzyloxy) phenyl]ethyl]isothiourea methanesulfonate] (10 microM), reduced A1254-induced [Ca2+]i increase....

  Abstract   Introduction: Neurodegeneration change is one of the hallmark symptoms of which Alzheimer’s disease (AD) can be modeled by β-amyloid injection into specific regions of brain. (-)-Epigallocatechin-3-gallate (EGCG) is a potent antioxidant agent that its role against oxidative stress and inflammation has been shown in prior studies. In the present study, we have wanted to determine wh...

BACKGROUND Prophylactic treatment regimens to prevent mother-to-child HIV transmission include protease inhibitors Lopinavir and Ritonavir. Lopinavir and Ritonavir have been reported to be able to induce intracellular oxidative stress in diverse cellular models, however scarce informations are available about protease inhibitor effects of in the central nervous system (CNS). In our study we eva...

Brain metastases derived from abdominal neuroblastoma are an uncommon complication of this tumour; however, an increase in their occurrence has recently been reported. In the present study, we have investigated the influence of factors derived from central nervous system glial cells on the proliferation of human neuroblastoma cells (SH-SY5Y) in vitro. Co-culture experiments show that a 24-h exp...

background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resisti...

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...

We employed transcriptome analysis of epidermal growth factor receptor related gene expression changes in cellular and animal models of Parkinson's disease (PD). We used a well-known Parkinsonian toxin 1-methyl-4-phenylpyridine (MPP⁺) to induce neuronal apoptosis in the human neuroblastoma SH-SY5Y cell line. The MPP⁺-treatment of SH-SY5Y cells was capable of inducing neuro-apoptosis, but it rem...