In order to test the hypothesis that serum ferritin below 60 micrograms/l is a good indicator of iron deficiency in patients with rheumatoid arthritis peroral iron was given to 67 patients with active rheumatoid arthritis over a three month period. A rise in haemoglobin concentration was taken as evidence of iron responsive anaemia. In anaemic patients serum ferritin below 60 micrograms/l was a...

The serum iron was determined in 60 normal subjects (38 males and 22 females) with a mean age of 32.0 and 36.2 years respectively. The plasma total iron-binding capacity (TIBC) was also determined in 54 of these subjects (32 males and 22 females). Serum iron and TIBC determinations were performed between 8.45 am and 9.15 am. The mean serum iron concentration is 138.3 +/- 4.3 mug/100 ml (SD +/- ...

ABSTRACT BACKGROUND In beta thalassaemia major multiple blood transfusions, ineffective erythropoiesis and increased gastrointestinal iron absorption lead to iron overload in the body. Iron overload impairs the immune system, placing patients at greater risk of infection and illness. Iron overload can be determined by serum ferritin measurement. OBJECTIVE The aim of the present study is to...

BACKGROUND Resistance to EPO therapy in hemodialysis (HD) patients is ascribed to inflammation and iron deficiency. Hepcidin, an antimicrobial peptide, is a key regulator of iron metabolism and synthesis of hepcidin is regulated by iron status and inflammation. The role of hepcidin in the pathogenesis of EPO-resistant anemia was assessed through measurement of serum pro-hepcidin in HD patients....

BACKGROUND Increased cellular iron exposure is associated with colorectal cancer (CRC) risk. Hepcidin, a liver peptide hormone, acts as the primary regulator of systemic iron status by blocking iron release from enterocytes into plasma. Concentrations are decreased during low iron status and increased during inflammation. The role of hepcidin and the factors influencing its regulation in CRC re...

Ferritin is a major iron storage protein. In case of iron deficiency, serum ferritin levels play a critical role in diagnosis and treatment.[1] Serum ferritin is an acute phase protein. Serum ferritin level is an indicator of body iron stores and may be normal or elevated in infective, inflammatory, or malignant disease.[2] Iron ions will be precipitated on the enamel surface as thin acid-resis...

Here, we presented new original data on the effects of iron depletion on the circulating lipid profile in B6HCV mice, a murine model of HCV-related dyslipidemia. Male adult B6HCV mice were subjected to non-invasive iron depletion by low iron diet. Serum iron concentration was assessed for evaluating the effects of the dietary iron depletion. Concentrations of circulating triglycerides, total ch...

Because of the large quantities of venous blood required, assays of total iron-binding capacity (TIBC) and serum iron are often omitted in diagnostic tests involving small children. As an alternative to the conventional method of assessing TIBC, we developed a micromethod based on flameless atomic absorption spectrophotometry (flameless AAS). Additionally, the standard microproc#{233}dure for d...

OBJECTIVE To examine the effects of iron deficiency and its treatment by iron supplementation or a high iron diet on fatigue and general health measures in women of childbearing age. DESIGN Randomised controlled trial to compare supplement and dietary treatment of iron deficiency. SUBJECTS 44 iron deficient (serum ferritin < 15 microg/L or serum ferritin 15-20 microg/L, plus two of the foll...

The Makey & Seal [(1976) Biochim. Biophys. Acta 453, 250-2561 method of polyacrylamide-gel electrophoresis in buffer containing 6M-urea was used to determine the distribution of iron between the N-terminal and C-terminal iron-binding sites of transferrin in human serum. In fresh serum the two sites are unequally occupied; there is preferential occupation of the N-terminal site. On incubation of...