Among 56 blood isolates of Vibrio species identified by sequencing analysis of 16S rRNA and rpoB genes, the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system correctly identified all isolates of Vibrio vulnificus (n = 20), V. parahaemolyticus (n = 2), and V. fluvialis (n = 1) but none of the isolates of serogroup non-O1/O139 (non-...

In Oregon and parts of Washington State, the incidence of serogroup B meningococcal disease increased substantially in 1994 (1). Multilocus enzyme electrophoresis (MEE) subtyping of N. meningitidis serogroup B strains collected in these areas during 1993 and 1994 suggested that these increases were due to a group of genetically related strains of the enzyme type-5 (ET-5) complex. ET-5 N. mening...

Immunohistology was used for the detection of Legionella pneumophila serogroup I in necropsy tissue. Study of pneumonic lung from the recent Stafford outbreak has shown that this technique has a high sensitivity. A retrospective postmortem examination showed that L pneumophila serogroup 1 was an unusual cause of pneumonia in Oxfordshire during the study period. L pneumophila serogroup 1 can be ...

conclusions the results showed that the extracted omvs were conformationally stable, and there were no pyrogenic determinants in omv. also the results showed that the omv elicited high level of specific antibodies against n. meningitidis serogroup a. these results indicate that the omv obtained here, can be used as a meningococcal vaccine after further investigation. materials and methods omvs ...

background: neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5–15% of adults. the aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type iv pilus biogenesis protein antige...

Neisseria meningitidis retains its ability to cause endemic and hiperendemic disease in human population living in any environment, as well as localized outbreaks and massive epidemics in civilians and military personnel. In Rio de Janeiro it has been reported in the 1990s as prolonged outbreak of serogroup B and at least one epidemic of serogroup C was well defined, both demanding quick action...

In this study, the serogroup and susceptibility patterns of Shigella and Salmonella spp. isolated from stool cultures were determined using standard laboratory procedures. Among the 76 Shigella isolates serogroup B (Sh. flexeneri) was the most prevalent species (54.0%) and among the 37 Salmonella strains serogroup B was also the most prevalent (81.1%). Antibiograms of Shigella and Salmonella sp...

A 79-year-old man was admitted to hospital from his nursing home for treatment of pneumonia, but died 7 days after admission. Legionella pneumonia was diagnosed after isolation of Legionella pneumophila serogroup-5 from sputum culture. The environment of the nursing home was investigated; only water specimens from the 24-hour bath were positive by culture for Legionella pneumophila serogroup-5....

Epidemic meningococcal infection is generally caused by single clones; whether nonepidemic increases in infection are clonal is unknown. We studied the molecular epidemiology of meningococcal infection during a period that the incidence increased in two age groups. Serogroup C and Y meningococcal isolates were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing. From 199...

Six different agglutinating antisera were obtained by immunizing rabbits with Formol-treated strains of Clostridium difficile. After appropriate absorption, these antisera were used to define six serogroups designated by the letters A, B, C, D, F, and G. Altogether, 315 strains of C. difficile from various origins were tested for slide agglutination by these antisera; 312 (99%) of them were agg...