A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.

Positive or negative electrical charges are frequently associated with biomolecules. When placed in an electric field, charged biomolecules move towards the electrode of opposite charge due to the phenomenon of electrostatic attraction. Electrophoresis is the separation of charged molecules in an applied electric field. The relative mobility of individual molecules depends on several factors. T...

By THOMAS G. FERRIS, ROBERT E. EASTERLING AND RICHARD E. BUDD T HE VARIOUS types of hemoglobins have been identified and reported by means of zone electrophoresis using supporting media such as filter paper,’ starch,2 agar,3 cellulose acetate,4 and combinations of these media. Recently a synthetic acrylamide gel ( Cyanogum 41#{176} ) has been investigated and found to have characteristics which...

INTRODUCTION The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N'-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. METHODS NIPAM polymer gel was prepared from non-electrophoresis grade BIS and the relaxation rate (R2) was measured by MR im...

In this paper, rapid identification of Bacillus anthracis genetic material in a portable microchip device for gel electrophoresis is presented. DNA fragments of virulence genes: cap, pag and sap of B. anthracis were separated and detected in a glass microchip within 4 minutes. Developed CCD-based detection setup, utilizing smart image processing, provided sensitive real-time detection of target...