AIM To study the effect of preputial washing on bacterial load, preservability and semen quality in Murrah buffalo bulls. MATERIALS AND METHODS A total of 36 collections of three Murrah buffalo bulls maintained at Artificial Breeding Research Centre, ICAR-National Dairy Research Institute, Karnal, were collected at weekly intervals from each bull without preputial washing and latter ejaculate...

The aim of the experiment was to determine the effect of potential antioxidants (adenosine, L-cysteine hydrochloride, ascorbic acid, magnesium fumarate and prolactin) supplementing the Biosolwens extender on semen survival time and sperm chromatin structure. The semen motility was examined every day and the susceptibility of sperm chromatin to denaturation was evaluated on collection day and da...

The survivability of caprine spermatozoa was compared in semen extenders prepared from the conventional egg-yolk citrate and goat-milk citrate. Motility was comparable in both sets of extenders during the first 24 hours post-extension when extended ejaculates were stored at either room temperature (28 O C) or refrigerator temperature (5 O C). Motility in goat milk citrate extenders at 5 O C was...

The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL-1 vitamin...

The aim of this study was to see how chilled sperm quality is affected by ejaculation frequency and its correlation seminal plasma composition in INRA 180 rams. Five rams were collected at high (HFE) low frequencies (LFE). For the frequency, three times on same day every days for 18 days. In collection, consecutive ejaculates once a week four weeks. Ejaculates 20-minute intervals either HFE or ...

The current research was designed to compare the effects of substitution egg yolk (EY) in ram extender with Arabic gum (AG) different concentration cooled or frozen semen collected by artificial vagina (AV) electro-ejaculator (EE) Noemi rams. Semen 2 times per week for 6 weeks from rams EE method and after a rest period weeks, samples were AV method. Tris either contained 15% EY (Control) 3%, 5...

The present study was designed to see whether adding glutathione to tris-citric egg yolk extender improves the quality of buffalo bull spermatozoa stored at 5°C for five days. Semen collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 5) was diluted (37C; 10 x 10 motile spermatozoa per ml) with extender having glutathione (0.0, 0.5, 1.0, 2.0 and 3.0mM). The extended semen was cooled fr...

the present study was aimed to test the efficacy of adding vitamins c or e to tris-fructose-egg yolk diluent to increase awassi ram sperm storage period at 5 ˚c. semen samples from six mature awassi rams were used in this study. the semen samples were diluted by tris-glucose-egg yolk. diluted semen sample was divided into three parts. the first part was added with 0.9 mg ml-1 vitamin c, the sec...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL(-1) vitamin C, the s...