INTRODUCTION Several options are available for preserving fertility before cytotoxic treatment, including ovarian tissue cryopreservation. Most reported surgical techniques include electrocoagulation. Our hypothesis is that avoidance of electrocoagulation may decrease ovarian cortex injury during cryopreservation procedures. CASE PRESENTATION We report a laparoscopic technique of whole-ovary ...

OBJECTIVES Human mesenchymal stem cells (MSCs) are efficacious in various cellular therapeutic applications and have been isolated from several tissues. Recent studies have reported that human tonsil tissue contains a new source of progenitor cells, potentially applicable for cell-based therapies. Information about the effects of donor age, long-term passage and cryopreservation are essential f...

Cryopreservation may have a variety of effects on embryo morphology, ranging from subtle damage on intracellular organelles, cytoplasm, or processes that can negatively affect normal cell development, to overtly blatant effects such as the lysis of one or more blastomeres. Many underlying factors can influence the outcome of cryopreservation, e.g. the quality of the oocyte or embryo itself will...

BACKGROUND Cryopreservation has been extensively used in assisted reproductive technology, agriculture and conservation programmes for endangered species. The literature reports largely positive results regarding the survival of frozen-thawed cells and clinical outcomes. Nonetheless, it is unclear whether or not cryopreservation of sperm, oocytes and embryos causes any disruption in their genet...

Recalcitrant seeds are characterized by desiccation and freezing sensitivity, short storage longevity. These physiological attributes obviate their ex situ conservation in conventional seed banks, where stored dry at sub-zero temperatures (typically, 15% relative humidity –20°C) for extended periods of time. Propagation plants field collections (e.g., botanical gardens, nurseries, arboretums) i...

Earlier there was found activity of glutamatergic ionotropic α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPAR) and N-methyl-D-aspartate receptors (NMDAR) disturbed after prolonged cryopreservation brain slices at temperature -10 оС. To eliminate cryodamage AMPAR NMDAR, the were encapsulated in special freezing solution (SFS). SFS consisted agar various concentrations (33, 44 50 %) ar...

In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation secures future reproduction, and insemination doses can be easily shipped. Processing of semen for cryopreservation can be done with minimal efforts and relatively low costs. In this chapter we describe the entire cryopreservation process for stallion and bull sperm including ...

Both sperm and embryo cryopreservation have become routine procedures in human assisted reproduction and oocyte cryopreservation is being introduced into clinical practice and is getting more and more widely used. Embryo cryopreservation has decreased the number of fresh embryo transfers and maximized the effectiveness of the IVF cycle. The data shows that women who had transfers of fresh and f...

Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in ge...

A cryopreservation condition for D-amino acid oxidase (DAAO)-overexpressing Escherichia coli (E. coli BL21(DE3)/pET-DAAO) was established. Ten percent was the optimum concentration of glycerol as a cryoprotectant, and its diffusion into stationary phase cells was superior to that into log cells. The results also showed that rather than fast cooling, a slow cooling method was appropriate to our ...