Recombinant human interferon alpha 2a (rhIFN α -2a) production and cell growth were monitored in a set of genetically modified E. coli strains (MSD1519, MSD1520, MSD 1521, MSD 1522, MSD 1523) producing rhIFN α -2a. The growth was followed at OD 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (PyMS) of cell biomass and recombinant protein production was determined...

ABSTRACT: Aerococcus viridans is an emerging pathogen for humans and livestock animals, mainly associated with genitourinary infections cases. Its occurrence in wild mammals has never been reported. The aim of this study was to determine the etiological agent clinical a case genital infection female African elephant (Loxodonta africana). Phylogenetic analysis antimicrobial susceptibility profil...

Background: Carnosine is a dipeptide molecule (?-alanyl-l-histidine) with anti-inflammatory, antioxidant, anti-glycation, and chelating properties. It used in exercise physiology as food supplement to increase performance; however, vitro evidence suggests that carnosine may exhibit anti-cancer Methods: In this study, we investigated the effect of on breast, ovarian, colon, leukemic cancer cell ...

Unconventional protein export/secretion (UPE/UPS), in contrast to the classical ER-Golgi-dependent of proteins with a leader sequence (signal peptide), employs multiple means release leaderless cargoes (and some special cases, sequence) extracellular space. By far, two major types UPE have been classified, vesicle-independent and vesicle-dependent UPE. In former, can directly tr...

The vascular network supporting the islets of Langerhans represents a highly specialised system arterioles, capillaries and venules. Several features islet vasculature (density fenestration capillaries) ensure rapid exchange nutrients hormones, which is central to islets' capacity control systemic metabolism via reciprocal changes insulin glucagon secretion. Here we discuss how in blood flow ma...

The role of glucagon-like peptide-1 (GLP-1) on gonadotropin-releasing hormone (GnRH) secretion was investigated in ovariectomised (OVX) ewes, which GnRH and luteinising (LH) had been restrained by treatment with oestrogen progesterone. Guide tubes for microinjection were placed above the median eminence (ME) animals allowed to recover 1 month. Jugular venous blood samples taken via cannulae at ...