An aminopeptidase was purrned 178-fold from an extract of Grijbla frondosa by ammonium sulfate precipitation and a series of column chromatographies on pheny)-Toyopearl, Sephadex G-25, and Mono-Q. The melecular mass of the enzyme was estimated te be 27 kDa and 30kDa by gel filtration and SDS-PAGE, respectively. The enzyme had an optimum pH of 8.5 and was stable between pH 6.e and pH 10.5, and i...

Catalysts for methanol synthesis from CO2 and H2 have been produced by two main methods: co-precipitation and supercritical anti-solvent (SAS) precipitation. These two methods are compared, along with the behaviour of copper supported on Zn, Mg, Mn, and Ce oxides. Although the SAS method produces initially active material with high Cu specific surface area, they appear to be unstable during rea...

The source of monomethylmercury (MMHg) in wet deposition is unknown. Volatilization of gaseous MMHg, evasion and demethylation of dimethylmercury, and methylation of Hg have been either proposed or tested unsuccessfully as potential sources. Here, we show that MMHg in precipitation, sampled across a wide geographical range in North America, is related positively to an operationally defined and ...

Abstract The kinetics and mechanism of the oxidative decolorization methylene blue (MB + ) as a cationic dye by persulfate using silver(I) catalyst in aqueous acidic medium were studied. effects different parameters like [H ], [Ag reactant concentration, micelles, temperatures investigated. kinetic data showed that [MB] obeyed pseudo‐first‐order taking large excess [S 2 O 8 2− ] over [MB ]. obs...

The amorphous platinum greens, which were synthesized from Pt ( N H s ) ~ ( H ~ O ) ~ (N03)2 or from Pt(NH3)2(H20)2(S04) and uridine, 5-bromouridine and ftorafur, were studied in the solid state by anomalous small angle x-ray scattering. The intensity curves exhibit a power-law dependence, which is likely to originate from large aggregates that are quite compact yet have rough surfaces. The sur...

Lethal and hemolytic toxins were purified by acetone precipitation, Sephadex G-50, CM-cellulose and CM-Sephadex column chromatography from the tentacles and bodies of the sea anemone Actinia equina. The isolated toxins, with a mol. wt of 19,000 determined by SDS-polyacrylamide gel electrophoresis, differed only slightly in amino acid composition and had a high tryptophan content. The isoelectri...

A highly sensitive enzyme-linked immunosorbent assay was used for the analysis of serum IgG reactivity against specific immunogenic Treponema pallidum proteins. Outer membrane treponemal proteins purified by preparative SDS-polyacrylamide gel electrophoresis were used as antigenic probes at concentrations as low as 100 ng per ml (5 ng per well in microtitre plates). Detection of anti-treponemal...

After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to limited efficiency of pellet reconstitution/solubilisation. As pelleted proteins tightly packed, they most often solubilised using chaotropic agents (e.g., guanidine hydrochloride or urea), detergents SDS), salts (NaCl) basic buffer (Tris). The aim this study was define optimise procedure...

Precipitation/dissolution reactions coupled with solute transport are modelled as a cellular automaton in which solute molecules perform a random walk on a regular lattice and react according to a local probabilistic rule. Stationary solid particles dissolve with a certain probability and, provided solid is already present or the solution is saturated, solute particles have a probability to pre...

OBJECTIVE This study was designed to investigate the protective effects of salidroside (SDS) via suppressing the expression of transforming growth factor-β1 (TGF-β1) in rat acute lung injury (ALI) induced by paraquat (PQ) and to explore the potential molecular mechanisms. METHODS A total of 90 male rats (190-210 g) were randomly and evenly divided into 9 groups: control group, PQ groups (4 gr...