We have developed a simple and effective animal model to study the distraction neurogenesis utilizing the sciatic nerve-lengthening technique in rats. The model allows macroscopic, physiological, and histological evaluation of the distraction site. Fourteen adult Harlan Sprague Dawley rats (300-350 g) were used in this study. A 10 mm segment of the right sciatic nerve of each animal in the nerv...

The use of sex hormones as potential neurosteroids capable of enhancing neuronal repair and functional recovery is gaining some momentum in the experimental literature. Two very interesting papers are presented in this issue of Restorative Neurology and Neuroscience outlining the effects of testosterone treatment upon anatomical and functional recovery after sciatic nerve crush injuries. The ar...

ObjectiveComprehensive functional, histomorphometrical and immunohistochemical assessment of sciatic nerve regeneration through an inside-out vein graft in rat. DesignExperimental in vivo study. AnimalsFiftyfour healthy male White Albino rats. ProceduresThe rats were divided into three experimental groups (n=18), randomly: Sham-operation (NC), Transected control (TC) and Inside-out vein graft (...

The goal of the present work was to develop minimally invasive imaging techniques to monitor local regeneration of peripheral nerves and to determine the extent of return to function of brain cortical regions associated with that nerve. The sciatic nerve crush model was applied to Sprague-Dawley rats and conventional histological staining for myelin, axons and cell architecture was carried out,...

Sensory axons in the adult spinal cord do not regenerate after injury. This is essentially because of inhibitory components in the damaged CNS, such as myelin-associated inhibitors and the glial scar. However, if the sciatic nerve is axotomized before injury of the dorsal column, injured axons can regenerate a short distance in the spinal cord. Here, we show that sciatic nerve transection resul...

BACKGROUND The aim of this study was to investigate the effects of negative pressure therapy in the regeneration of the rabbit sciatic nerve using vacuum assisted closure (VAC). MATERIAL AND METHODS Thirty male New Zealand white rabbits underwent surgical injury of the sciatic nerve, followed by negative pressure therapy using vacuum assisted closure (VAC), in three treatment groups: Group A: 0...

Introduction: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can increase axonal regeneration in peripheral nerve injuries. Based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury . We pursued to evaluate the effects of BMSCs and SCs co-transplant...

In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each group), in which the nerve was either not treated (control group) or treated with chitosan conduit, h...

BACKGROUND Autologous nerve grafts are used to bridge peripheral nerve defects. Limited sources and donor site morbidity are the major problems with peripheral nerve grafts. Although various types of autologous grafts such as arteries, veins and muscles have been recommended, an ideal conduit has not yet been described. AIMS To investigate the effectiveness of a small intestinal conduit for p...

background: considering the common origin of skin and peripheral nervous system, a tube of dermal layer of skin hypothetically can be an ideal conduit for nerve reconstruction. an experimental study performed to evaluate the nerve regeneration of efficacy into a dermal tube. methods: sixty male wistar rats were used. a 10 mm gap was produced in right sciatic nerves. in group a the autogenous ne...