The authors present the application of wet SEM for histopathological assessment, a technology for imaging fully hydrated samples at atmospheric pressure in a scanning electron microscope (SEM). Both transmission and scanning electron microscopy techniques usually require long and complex sample preparation of the tissues. In marked contrast, a rapid preparation of tissues is described for evalu...

We present an integrated light-electron microscope in which an inverted high-NA objective lens is positioned inside a scanning electron microscope (SEM). The SEM objective lens and the light objective lens have a common axis and focal plane, allowing high-resolution optical microscopy and scanning electron microscopy on the same area of a sample simultaneously. Components for light illumination...

This paper reports on a study of the scanning electron microscope (SEM) Moiré method. Tests were carried out by rotating the specimen grating slightly with respect to the electron scanning raster lines, to verify that the Moiré images captured were really due to the interference between specimen and reference gratings. The experimental results coincided well with the calculated theoretical valu...

The scanning electron microscope (SEM) provides an ideal system for studying the three-dimensional surface structure of biological materials . Our previous work on the protozoan Opalina showed that instantaneous fixation, combined with critical point drying, faithfully preserved the pattern of ciliary coordination and the form of ciliary beat for scanning electron microscopy (1, 2) . I report h...

Abstract Background The influence of ? -glucan on the human gut microbiota is closely related to physicochemical structure -glucan. We purified a homogeneous water-soluble polysaccharide from Tibetan hull-less barley 25 and studied its in vitro fermentation profile. Results Analysis by gas chromatography (GC), Fourier-transformed infrared (FT-IR), nuclear magnetic resonance (NMR), scanning elec...

nanocrystals of zno were prepared by microwave irradiation using zn (ii) acetate and triethanol amin (tea) as the starting materials and water as solvent. the nanocrystals of zno with hexagonal (wurtzite) structure were characterized by x-ray powder diffraction (xrd), transmission electron microscopy (tem), scanning electron microscopy (sem), uv-vis absorption and ftir spectroscopy techniques.