BACKGROUND One of the most important producers of high quality industrial enzymes is the Gram-positive bacterium, Bacillus subtilis (B. Subtilis). One major limitation that hinders the wide application of B. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. In this study, homologus recombination technique was used to knock out its p...

A total of 28 samples were processed, of which 20 isolates were identified as phosphate solubilizing bacteria. Among 20 isolates, 12 (60 %) belonged to B. subtilis, 3 (15%) were B. brevis, 3 (15%) belonged to Pseudomonas aeruginosa and 2 (10 %) were identified as Enterobacter aerogenes. All the isolates exhibited phosphate solubilization (1.009 μg/ml to 1.609 μg/ml), produced Indole acetic acid...

A study was carried out to evaluate the probiotic activity of Bacillus subtilis G1 isolated from fermented pickles in growth performance and disease resistance of Hemibagrus nemurus fingerlings at Universiti Putra Malaysia. The probiotic was mixed in feed at doses of 0 (C, control), 3 x 109 (T1) 3 x 107 (T2) and 3 x 105 (T3) cfu g-1 and fed to the catfish fingerlings for nine weeks. Results sho...

Abstract Background Powdery mildew was found in most of the sunflower fields Egypt, causing severe yellowing blade, petiole, stem and calyx, as well a considerable defoliation during summer season 2018. Out fungal mycelium from infected leaves, collected four Egyptian Governorates (Kafr El-Sheikh, Gharbia, Giza El-Beheira), five isolates powdery pathogen were obtained identified using morpholog...

Bacillus subtilis strain TrigoCor 1448 was grown on wheat middlings in 0.5-l solid-state fermentation (SSF) bioreactors for the production of an antifungal biological control agent. Total antifungal activity was quantified using a 96-well microplate bioassay against the plant pathogen Fusarium oxysporum f. sp. melonis. The experimental design for process optimization consisted of a 2(6-1) fract...

Bacillus subtilis is a widespread and diverse bacterium t exhibits a remarkable intraspecific diversity of the ComQXPA quorum-sensing (QS) system. This manifests in the existence of distinct communication groups (pherotypes) that can efficiently communicate within a group, but not between groups. Similar QS diversity was also found in other bacterial species, and its ecological and evolutionary...

BACKGROUND Technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. Protein secretion can be achieved via general secretion (Sec) pathway. Specific sequences, signal peptides (SPs), are necessary to direct the target protein into the translocation machinery. For example, >150 Sec-specific SPs have bee...

ABSTRACT The biological control, thinking about the integrated management, has been inserted with other management techniques to disease such as soybean anthracnose. aims of this work were verify action Trichoderma and Bacillus isolates in induction resistance mechanisms anthracnose a function seed treatment. statistical design was entirely randomised, 5 x 2 (agent species sampling times) facto...