Endothelial HMEC-1 cells incubated with pro-inflammatory cytokine TNF-α for 6 and 24 hours were studied as a model of inflammation using Raman imaging. Striking changes in distribution, composition and concentration of cellular lipids were observed after exposure to TNF-α compared to the control. In particular, 3D Raman imaging revealed a significant increase in the amount of lipid entities for...

P. TEIXEIRA, H. CASTRO AND R . KIRBY. 1996. Membrane fatty acids of Lactobacillus hulgaricus were analysed by gas-liquid chromatography before and after spray drying. T h e ratio unsaturated/saturated fatty acids decreased following spray drying, indicating the formation of lesions in cellular lipid-containing structures. The same method was used to analyse membrane lipids of Lact. hulgaricus d...

The fluorescence kinetics of 1,6-diphenyl-1,3,5-hexatriene (DPH) dissolved in cyclohexane was investigated as a function of temperature, concentration and 355 nm excitation pulse energy. At concentrations above 2.5 μM and excitation energies above 1 mJ a long-lived, very intense emission, which appears within less than 5 ns and lasts up to 70 ns, is observed. During the first 50 ns the decay do...

Whether naturally- or artificially-induced due to human activities, decreasing increasing the suction in multiphase-fluid-saturated porous materials can lead enormous changes their thermo-hydromechanical properties. In this, both mathematical description and numerical modeling of coupled problem present a challenging task. The following contribution considers instance related micro-cryosuction-...

in this research, a semi empirical approach has been suggested for calculating the activation energyof unimolecular thermal decomposition of vinyl ethers yielding saturated products. the calculationprocedure is based on the use of molecular mechanics (mm) methods. these methods which involvethe construction of the transition state for a molecule mainly consider the formation of a “hydrogenbridg...

The effect of transferrins on hydroxyl radical formation from the superoxide anion and hydrogen peroxide generated by the xanthine-xanthine oxidase system has been studied by EPR using 5,5-dimethyl-1-pyrroline N-oxide as a spin trap. Neither diferriclactoferrin nor diferrictransferrin were found capable of promoting hydroxyl radical formation via the Haber-Weiss reaction even in the presence of...

The dielectric constant of a partially saturated sandstone varies as a function of the level of water saturation. Experimental data indicate that rock/water interaction, at low saturations, has a large effect on the measured dielectric response. To theoretically predict the dielectric constant of the rock/water/air system, this rock/water interaction must be accounted for by including the effec...

Natural gas hydrates (NGHs) are solid non-stoichiometric compounds often regarded as a next generation energy source. Successful commercialization of NGH is curtailed by lack of efficient and safe technology for generation, dissociation, storage and transportation. The present work studied the influence of environment compatible biosurfactant on gas hydrate formation. Biosurfactant was produced...

Silica scaling is probably the principal factor limiting the extraction of energy from high temperature geothermal systems. Silica exists in a number of polymorphs from quartz through to opal A (or amorphous silica). The thermodynamics of the solubility of quartz and amorphous silica with variations in temperature, salt content, pH and pressure are discussed. However, in silica saturated geothe...

soil hydrau-physical data are important for many hydrological modelings. they are the main variables controllingthe key processes such as water and chemicals movement and transport in the soil profiles. this study was conductedin order to analyze the spatial distribution of selected soil hydrau-physcial characteristics including infiltration rate(ir), saturated hydraulic conductivity (ks), bulk...