The recent cloning of chicken genes coding for interleukins, chemokines, and other proteins involved in immune regulation and inflammation allowed us to analyze their expression during infection with Eimeria. The expression levels of different genes in jejunal and cecal RNA extracts isolated from uninfected chickens and chickens infected with Eimeria maxima or E. tenella were measured using a p...

Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of...

Immunostimulating complexes (ISCOMs) are unique, multimolecular structures formed by encapsulating antigens, lipids, and triterpene saponins of plant origin, and are an effective delivery system for various kinds of antigens. The uses of ISCOMs formulated with saponins from plants collected in Kazakhstan, with antigens from the poultry coccidian parasite Eimeria tenella, were evaluated for thei...

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly. Discovery of the PIP5K gene in Eimeria tenella may shed light on its role in the biology of this avian protozoan, and afford further understanding of the cell-host interactio...

The aim of present investigation was to study the virulence of coccidiosis in Kadaknath birds, experimentally infected with E. tenella by OPG count, oocyst index and caecal lesions scoring. Sixty one-day old unsexed Kadaknath birds were randomly divided into a control, T1 and T2 groups comprising twenty chicks under each group. At d 21 of age, birds of T1 and T2 groups were individually inocula...

An outbreak of fever associated with myalgia and myositis occurred in 2012 among 89 of 92 college students and teachers who visited Pangkor Island, Malaysia. The Sarcocystis nesbitti 18S rRNA gene and sarcocysts were obtained from muscle tissues of 2 students. Our findings indicate emergence of S. nesbitti infections in humans in Malaysia.

Development of an in vitro Eimeria (E.) tenella model could be valuable as a tool for vaccine, coccidiostats or molecular biology research. 1.0 × 10,000 sporozoites per 0.1 mL were inoculated into the allantoic cavity of ten-day-old chicken embryos. The complete life-cycle of E. tenella was accomplished in eight-nine days at 37 °C and 70% humidity. The addition of 100 U insulin to the embryos c...

background: to control avian coccidiosis with drug-independent strategy effec-tively and safely, multivalent hyperimmune egg yolk immunoglobulin (igy) was prepared and its ability to protect against eimeria tenella infection was evaluated. methods: hens were orally immunized with live oocysts of 5 species of eimeria for six times, antibody titers in serum and yolk were monitored by indirect enz...

BACKGROUND Through 2 international traveler-focused surveillance networks (GeoSentinel and TropNet), we identified and investigated a large outbreak of acute muscular sarcocystosis (AMS), a rarely reported zoonosis caused by a protozoan parasite of the genus Sarcocystis, associated with travel to Tioman Island, Malaysia, during 2011-2012. METHODS Clinicians reporting patients with suspected A...

sarcocystis is a coccidia protozoan develops obligatory in two hosts consisting of the herbivores as an intermediate host (prey) and the carnivores as a host (predator). depend on species, the size of cysts and structure of cyst wall in intermediate hosts are different. the ultrastructure of sarcocystis cysts (sarcocysts) could be a good indication for diagnosis of the parasite species. in the ...