نتایج جستجو برای: rt pcr nested pcr

تعداد نتایج: 222624  

Journal: :Poultry science 2008
D A Roussan W S Totanji G Y Khawaldeh

Infectious bronchitis virus (IBV) causes respiratory disease in chickens all over the world. Infectious bronchitis virus has many serotypes that do not confer cross protection against each other. The current study was designed to know which IBV types were circulating in Jordanian broiler chickens. Tracheal swabs from 175 broiler flocks at the acute phase of respiratory disease were collected. T...

Journal: :Journal of clinical microbiology 2005
Roberta Vieira de Morais Bronzoni Flávia Graciela Baleotti Rita Maria Ribeiro Nogueira Márcio Nunes Luiz Tadeu Moraes Figueiredo

A new approach was developed for the rapid detection and identification of Brazilian alphaviruses and flaviviruses. The methodology involves the genus-specific detection of Alphavirus and Flavivirus by a duplex reverse transcription-PCR (D-RT-PCR), followed by multiplex nested PCR (M-N-PCR) or nested PCR (N-PCR) assays for species-specific identification. By this protocol, 25 arboviruses were s...

Journal: :Arquivos de neuro-psiquiatria 2016
Sérgio Monteiro de Almeida Sônia Mara Raboni Meri Bordignon Nogueira Luine R Renaud Vidal

The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics o...

Journal: :Medical principles and practice : international journal of the Kuwait University, Health Science Centre 2006
Hanady A Amoudy Mariam B Al-Turab Abu S Mustafa

OBJECTIVE To identify transcriptionally active open reading frames (ORFs), predicted by bioinformatics, within RD1 genomic segment of Mycobacterium tuberculosis using reverse transcription-polymerase chain reaction (RT-PCR). MATERIALS AND METHODS M. tuberculosis H37Rv was grown in Middlebrook 7H9 medium for 8 weeks and total RNA was isolated using standard procedures. The cDNA was synthesized...

Journal: :The Southeast Asian journal of tropical medicine and public health 2015
Uraiwan Intamaso Poramin Boonbanjong Saitharn Wisunthorn Nirun Ong-in Boonyarat Keawpo Witthaya Poomipak

Abstract. An efficient and rapid virus detection method is required for routine monitoring and risk assessment in food products. A duplex RT-coupled nested PCR method was developed to detect the simultaneous presence of hepatitis A virus (HAV) and rotavirus in commercial oysters from the eastern coast of Thailand. Primers were designed to amplify HAV VP4 and rotavirus VP7 genes. Although excess...

Journal: :Journal of molecular endocrinology 2002
S J Roddiger J Bojunga V Klee M Stanisch H Renneberg E Lindhorst K H Usadel K Kusterer P M Schumm-Draeger R Kurek

The aim of this study was to evaluate thyroid peroxidase (TPO) mRNA expression in peripheral blood of patients with benign and malignant thyroid disease. Included were 120 thyroid cancer patients, 85 patients with goitre or Graves' disease (GD) and 54 healthy volunteers. TPO mRNA expression was analysed in peripheral blood by nested RT-PCR. In cancer patients, RT-PCR results were compared with ...

Journal: :Journal of clinical microbiology 2001
P Y Shi E B Kauffman P Ren A Felton J H Tai A P Dupuis S A Jones K A Ngo D C Nicholas J Maffei G D Ebel K A Bernard L D Kramer

The recent outbreaks of West Nile virus (WNV) in the northeastern United States and other regions of the world have made it essential to develop an efficient protocol for surveillance of WNV. In the present report, we describe a high-throughput procedure that combines automated RNA extraction, amplification, and detection of WNV RNA. The procedure analyzed 96 samples in approximately 4.5 h. A r...

Journal: :Anticancer research 2006
M J Ribal L Mengual M Marín F Algaba E Ars P L Fernández R Oliva H Villavicencio A Alcaraz

BACKGROUND The aim of this study was to analyze whether the CK20 reverse transcriptase polymerase chain reaction (RT-PCR) is suitable for detecting circulating tumor cells and residual tumor cells in lymph nodes, in patients with muscle invasive transitional cell carcinoma (TCC) of the bladder, and to compare these results with standard histological staging. PATIENTS AND METHODS The nested RT...

Journal: :modares journal of medical sciences: pathobiology 2010
shahab falahi mehrdad ravanshad azra kenar koohi

objective: polymerase chain reaction (pcr) is one of the most important progress in the field of molecular biology and diagnosis. despite simplicity in concept, the reaction needs complex interaction between target sequence, primers, dntps and dna polymerase for a successful amplification and diagnosis. for the detection of rna viruses highly sensetive and specific technique is required. hence ...

2012
Mohammad Khezri Seyed Mahmoud Azimi

Aim: The objective of this study was conducted to determine the seroprevalence and S7 gene characterization of bluetongue virus (BTV) of sheep in the West of Iran, during 2007-2008. Materials and Methods: A total 372 sheep blood samples were collected from known seropositive regions in the West of Iran. Anti-BTV antibodies were detected in the serum samples by group specific, C-ELISA. Extractio...

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