Vera C. Terra1, Américo C. Sakamoto1, Hélio R Machado1 and Fulvio A. Scorza2 1Centro de Cirurgia de Epilepsia (CIREP). Departamento de Neurociências e Ciências do Comportamento. Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. Ribeirão Preto, São Paulo, 2Disciplina de Neurologia Experimental. Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Pa...

Ricin, the highly toxic, hemagglutinating protein of the castor bean, has always been of great interest to the chemist and the immunologist (l-4), partly because it, may readily be obta.ined in considerable quantities. Earlier methods of preparation were carried out before the development of modern physicochemical and immunochemical methods for characterizing proteins and data are therefore lac...

The short arms of five human acrocentric chromosomes contain ribosomal gene (rDNA) clusters where numerous mini-nucleoli arise at the exit of mitosis. These small nucleoli tend to coalesce into one or a few large nucleoli during interphase by unknown mechanisms. Here, we demonstrate that the N- and C-terminal domains of a nucleolar protein, hNopp140, bound respectively to alpha-satellite arrays...

BACKGROUND The present study assessed microbial contamination in Italian dental surgeries. METHODS An evaluation of water, air and surface microbial contamination in 102 dental units was carried out in eight Italian cities. RESULTS The findings showed water microbial contamination in all the dental surgeries; the proportion of water samples with microbial levels above those recommended decr...

background: in this study, erythromycin resistance isolates from the students with aged 12-15 yr old were studied and the mutations in the 23s rrna were identified by sequencing. methods: throat samples of 327 students with 12-15 years old from 3 schools of tehran were cultured on the selective strep­tococ­cus pyogenes medium and 23 strains were primarily selected as s. pyogenes . results: all ...

background: to differentiate sarcocystis macro-cyst-forming species in slaughtered sheep in babol area, mazandaran province, sequence analysis of 18s rrna gene was performed. methods: overall, 150 slaughtered sheep were examined macroscopically in slaughterhouse, babol and intra-abdominal and diaphragm muscles tissues infected with macro-cyst of sarcocystis spp. were collected in 2013. one macr...

background : cryptosporidium is an intracellular apicomplexan parasite that infects a wide range of vertebrates including humans. cryptosporidiosis is a major cause of diarrhea in children with and without human immunodeficiency virus (hiv) infection in developing countries. more recently, the molecular methods for identification of morphologically indistin­guishable species have been developed...

The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of mutations in one operon is diluted by the six remaining wild-type copies. To create a tool useful for manipulating rRNA, we sequentially inactivated from one to all seven of these operons with deletion...

Three small nucleolar RNAs (snoRNAs), E1, E2 and E3, have been described that have unique sequences and interact directly with unique segments of pre-rRNA in vivo. In this report, injection of antisense oligodeoxynucleotides into Xenopus laevis oocytes was used to target the specific degradation of these snoRNAs. Specific disruptions of pre-rRNA processing were then observed, which were reverse...