Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of diff...

A suitable protocol for micropropagation of Casuarina hybrid, Casuarina equisetifolia L. 9 Casuarina glauca Sieber ex Spreng (C. e. 9 C. g.), was developed. When seeds without seed coats were cultured on 4 germination media, the optimal seed germination percentage (91%) was obtained on 0.8% agar solidified water medium. Shoot multiplication was achieved by culturing 2-cm long epicotyls, excised...

Abstract—Developing our knowledge of when pineapple roots grow can lead to improved water, fertilizer applications, and more precise culture management. This paper presents current understanding of morphological traits in pineapple roots, highlighting studies using incubation periods and various solid MS media treated with different sucrose concentrations and pH, which directly assess in vitro ...

In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials high qualities. Appropriate culture media formulation needed for shoot growth rooting stage. This experiment aimed study effects IAA organic supplements on in rooting. Explants were subjected MS medium with various supplements, a factorial arrangement completely randomized desig...

In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2...

Hybrids of Solanum melongena and S. aethiopicum are of interest as rootstocks of eggplant, as they are highly vigorous and can incorporate resistance to several diseases. However, hybridization between both species is difficult. Therefore, protocols for in vitro culture are of great interest for their micropropagation and biotechnological breeding. We assessed the organogenesis response from le...

Cyperus scariosus R.Br is a valuable multipurpose medicinal herb, which belongs to family Cyperaceae. It possesses astringent, anti-inflammatory, diaphoretic, diuretic, cordial and stomach ache properties. An attempt has been made to study in vitro regeneration, floral bud induction, multiple shooting and multiple rooting has been achieved via healthy, moderate axillary buds as explants. The ax...

This study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitr...

Morphogenic potential of cabbage cv. Pride of India, for multiple shoot induction was tested under in vitro conditions using cotyledon and hypocotyl explants. Aseptically grown seven to nine days old seedlings of cabbage were used as source of explants for reproducible plant regeneration studies. Forty different concentrations and combinations of TDZ (alone), TDZ with adenine, TDZ with NAA and ...

The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (...