Several peptides contained within the amino acid sequence -Glu-Gly-Asn-Pro-Tyr-Val-Pro-Val-His-Phe-Asp-Ala-SerVal-OH at the carboxyl end of ribonuclease A were synthesized in which phenylalanine-120 was replaced by leucine, isoleucine, or tryptophan: [Leul*O]-RNase 111-124, [Ile120]RNase 111-124, [Trp’*Ol-RNase 111-124, ple*20]-RNase 113124, [Leul*O]-RNase 115-124, ple120]-RNase 115-124, [Trpl*...

Bacillus subtilis possesses three essential enzymes thought to be involved in mRNA decay to varying degrees, namely RNase Y, RNase J1, and RNase III. Using recently developed high-resolution tiling arrays, we examined the effect of depletion of each of these enzymes on RNA abundance over the whole genome. The data are consistent with a model in which the degradation of a significant number of t...

RNase T is one of eight distinct 3'-->5' exoribonucleases present in Escherichia coli. The enzyme plays an important role in stable RNA metabolism, including tRNA end turnover and 3' maturation of most stable RNAs because it is the only RNase that can efficiently remove residues near a double-stranded (ds) stem. In the course of study of its specificity and mechanism, we found that RNase T also...

In gram-positive bacteria, RNase J1, RNase J2 and RNase Y are thought to be major contributors to mRNA degradation and maturation. In Staphylococcus aureus, RNase Y activity is restricted to regulating the mRNA decay of only certain transcripts. Here the saePQRS operon was used as a model to analyze RNase Y specificity in living cells. A RNase Y cleavage site is located in an intergenic region ...

Serum ribonuclease (RNase) levels were measured in 29 patients with leukemia at various times during their illness and compared with 54 normal controls and 28 nonleukemic patients on chronic hemodialysis. Eighty-three % of the leukemic patients examined before therapy and with normal rates of glomerular filtration (as defined by creatinine clear ance greater than 60 ml/min) had RNase levels tha...

Plants, animals, bacteria, and Archaea all have evolved mechanisms to cope with environmental or cellular stress. Bacterial cells respond to the stress of DNA damage by activation of the SOS response, the canonical RecA/LexA-dependent signal transduction pathway that transcriptionally derepresses a multiplicity of genes-leading to transient arrest of cell division and initiation of DNA repair. ...

Among the Rosaceae species, the gametophytic self-incompatibility (GSI) is controlled by a single multi-allelic S locus, which is composed of the pistil-S and pollen-S genes. The pistil-S gene encodes a polymorphic ribonuclease (S-RNase), which is essential for identifying self-pollen. However, the S-RNase system has not been fully characterized. In this study, the self-S-RNase inhibited the Ca...

BACKGROUND Ribonuclease-L (RNase-L) was known to be a ubiquitous enzyme involved in several cellular functions, especially innate immunity. It was recently shown to participate in adipogenesis in rodents. Here, we developed a method to measure serum levels of RNase-L and analyzed the relationship between RNase-L and metabolic syndrome (MetS). METHODS A total of 396 subjects were recruited fro...

It has been suggested that Alba, a well-established chromatin protein in Archaea, is also a subunit of the archaeal RNase P holoenzyme, based on the observation that the homolog of this protein in humans has been shown to be associated with RNase P activity. Using the same biochemical methods we used previously to show that four other proteins homologous to eukaryotic RNase P proteins are bona ...

The proteolysis of bovine pancreatic ribonuclease by subtilisin has been shown to involve the formation of an enzymically active intermediate which is further degraded to inactive products (1, 2). This paper describes the preparation of the modified active ribonuclease, RNase (S)l and its fractionation into two components, a peptide (S-peptide) and a protein (S-protein). Neither S-peptide nor S...