نتایج جستجو برای: rna seq

تعداد نتایج: 256145  

Journal: :Nature Reviews Genetics 2013

2017
Yong-Wei Yan Bin Zou Ting Zhu Wael N Hozzein Zhe-Xue Quan

RNA-seq-based SSU (small subunit) rRNA (ribosomal RNA) analysis has provided a better understanding of potentially active microbial community within environments. However, for RNA-seq library construction, high quantities of purified RNA are typically required. We propose a modified RNA-seq method for SSU rRNA-based microbial community analysis that depends on the direct ligation of a 5' adapto...

Journal: :Current Protocols in Molecular Biology 2018

2017
Nathan T. Johnson Andi Dhroso Katelyn J. Hughes Dmitry Korkin

The extent to which the genes are expressed in the cell can be simplistically defined as a function of one or more factors of the environment, lifestyle, and genetics. RNA sequencing (RNA-Seq) is becoming a prevalent approach to quantify gene expression, and is expected to gain better insights to a number of biological and biomedical questions, compared to the DNA microarrays. Most importantly,...

2016
Konstantin Okonechnikov

The transcriptome plays an important role in the life of a cell. Detailed analysis of the transcriptome enables interpretation of its structure and functionality. High throughput sequencing technology signi cantly enhanced the understanding of transcriptome activity. The RNA-sequencing process currently provides the most accurate estimation of gene expression levels. Moreover, RNA-seq allows de...

Journal: :Bioinformatics 2011

2016
Stefan Canzar Karlynn E. Neu Qingming Tang Patrick C. Wilson Aly A. Khan

MOTIVATION The B-cell receptor enables individual B cells to identify diverse antigens, including bacterial and viral proteins. While advances in RNA-sequencing (RNA-seq) have enabled high throughput profiling of transcript expression in single cells, the unique task of assembling the full-length heavy and light chain sequences from single cell RNA-seq (scRNA-seq) in B cells has been largely un...

Journal: :Journal of virology 2013
Joseph D Khoury Nizar M Tannir Michelle D Williams Yunxin Chen Hui Yao Jianping Zhang Erika J Thompson Funda Meric-Bernstam L Jeffrey Medeiros John N Weinstein Xiaoping Su

Elucidation of tumor-DNA virus associations in many cancer types has enhanced our knowledge of fundamental oncogenesis mechanisms and provided a basis for cancer prevention initiatives. RNA-Seq is a novel tool to comprehensively assess such associations. We interrogated RNA-Seq data from 3,775 malignant neoplasms in The Cancer Genome Atlas database for the presence of viral sequences. Viral int...

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