Four strains of RHDV assigned as haemagglutinating (Vt97 and Hartmannsdorf) and non-haemagglutinating (Pv97 and 9905) antigenic variants were examined for dynamic changes in the values of white and red blood cells indexes. The study showed differences among strains examined that were not depending on haemagglutination property.

Interleukin-2 (IL-2) and T cell subpopulations were evaluated in children with rheumatic heart disease (RHD). Three groups were included: 13 patients with active RHD, 12 with non-active RHD, and 14 control children. Serum IL-2 and T cell subpopulations were measured by radioimmunoassay and monoclonal antibodies respectively. Patients with active RHD showed a significant increase in IL-2 concent...

OBJECTIVE To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested f...

Background Comparing the ABO and RhD group of a recipient's current pre-transfusion sample against their historical group is an important means of detecting wrong blood in tube (WBIT) errors. This study investigated the utility of using the donor ABO and RhD group as the historical check for recipients. Methods A single database stores serological information on blood donors, pregnant women, ...

The Rh system is the most polymorphic and immunogenic for all blood group systems. Currently more than 49 antigens were identified with five major antigens D, C, c, E, e. Knowledge of the Rh system's molecular basis, since its first cloning 17 years ago, allowed to understand the mechanism of Rh-negative phenotype and the variants of antigens as RHD and RHCE. Deletions, gene rearrangements and ...

Rhesus-positive and Rhesus-negative persons differ in the presence-absence of highly immunogenic RhD protein on the erythrocyte membrane. The biological function of the RhD molecule is unknown. Its structure suggests that the molecular complex with RhD protein transports NH3 or CO2 molecules across the erythrocyte cell membrane. Some data indicate that RhD positive and RhD negative subjects dif...

Background: Rheumatic heart disease remains a leading cause of morbidity and mortality among young adults in Uganda. Genetic susceptibility through the major histocompatibility class II human leucocyte antigens has been strongly associated with RHD. In Uganda and much of sub Saharan Africa there is lack of data on genetic susceptibility to RHD. Objective: The objective of this study was to comp...

Medea factors or genes are maternal-effects mechanisms, found in many species, in which the mother’s body selectively kills embryos of a certain genotype. Humans have a similar genetic mechanism, the gene RHD which produces Rh-factor involved in blood type. Recently I proposed that RHD acts as a maternal-effects gene that determines handedness (i.e., right handed or non-right handed) in individ...

Rhesus D category VI (DVI) is the clinically most important partial D. DVI red blood cells were assumed to possess very low RhD antigen density and to be caused by two RHD-CE-D hybrid alleles. Because there was no population-based work-up, we screened three populations in central Europe for DVI. Twenty-six DVI samples were detected and examined by exon-specific RHD polymerase chain reaction wit...