Dysfunctions of MeCP2 protein lead to various neurological disorders such as Rett syndrome and Autism. The exact functions of MeCP2 protein is still far from clear. At a molecular level, there exist contradictory data. MeCP2 protein is considered a single immunoreactive band around 75 kDa by western-blot analysis but several reports have revealed the existence of multiple MeCP2 immunoreactive b...

This paper describes a machine learning method, called Regression on Feature Projections (RFP), for predicting a real-valued target feature, given the values of multiple predictive features. In RFP training is based on simply storing the projections of the training instances on each feature separately. Prediction of the target value for a query point is obtained through two averaging procedures...

Multipotent neural stem cells (NSCs) can be isolated from various regions of the adult brain and propagated in vitro. Recent reports have suggested spontaneous fusion events among NSCs when grown as free-floating neurospheres that may affect the genetic composition of NSC cultures. We used adult NSCs expressing either red fluorescent protein (RFP) or green fluorescent protein (GFP) to analyze t...

Biofilms of Candida albicans include both yeast cells and hyphae. Prior studies indicated that a zap1Δ/Δ mutant, defective in zinc regulator Zap1, has increased accumulation of yeast cells in biofilms. This altered yeast-hypha balance may arise from internal regulatory alterations or from an effect on the production of diffusible quorum-sensing (QS) molecules. Here, we develop biosensor reporte...

We propose a new cell biology where the behavior of cells can be visualized in the living animal. An example of the new cell biology is dual-color fluorescence imaging using red fluorescent protein (RFP)-expressing tumors transplanted in green fluorescent protein (GFP)-expressing transgenic mice. These models show with great clarity the details of tumor-stroma interactions and especially tumor-...

We have recently developed a new model of tumor-host interaction consisting of red fluorescent protein (RFP)-expressing tumors transplanted to green fluorescent protein (GFP) transgenic mice expressing GFP in all tissues. We have previously shown that this model allows the whole-body imaging of tumor growth and metastasis and the visualization of host cells and structures including blood vessel...

Rapid Freezing Prototyping (RFP) with water is a novel solid freeform fabrication technique that can generate three-dimensional ice objects by depositing and rapidly freezing water layer by layer. The support where necessary is made of brine whose. freezing point is lower than. pure water. After building the part, the support can be removed by utilizing the melting temperature difference betwee...

Optical emission computed tomography (optical-ECT) is a technique for imaging the three-dimensional (3D) distribution of fluorescent probes in biological tissue specimens with high contrast and spatial resolution. In optical-ECT, functional information can be imaged by (i) systemic application of functional labels (e.g. fluorophore labelled proteins) and/or (ii) endogenous expression of fluores...