The binding of labeled hyaluronate to the surface of Simian virus 40-transformed 3T3 cells was studied as a function of 1) pH, 2) ionic strength, 3) temperature, and 4) molecular weight of the hyaluronate. Binding occurred over a wide range of pH values with optima at pH 7 and at less than pH 4. Binding at low pH was eliminated at high ionic strength whereas that at physiological pH was enhance...

Cyanobacteriochromes are linear tetrapyrrole-binding photoreceptors produced by cyanobacteria. Their chromophore-binding GAF domains categorized into many lineages. Among them, dual Cys-type cyanobacteriochrome possessing not only a highly conserved ‘first Cys' but also ‘second found from multiple The first Cys stably attaches to C31 of the A-ring, while second mostly shows reversible ligation ...

Design of irreversible inhibitors is an emerging and relatively less explored strategy for the design of protein kinase inhibitors. In this paper, we present a computational workflow that was specifically conceived to assist such design. The workflow takes the form of a multi-step procedure that includes: the creation of a database of already known reversible inhibitors of protein kinases, the ...

This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as the green fluorescent protein, enabling fluorescent labeling of proteins in a reversible and specific manner through the reversible binding and activation of a cell-permeant and nontoxic fluorogenic ligand (a so-called fluorogen). A unique fluorogen activation...

A critical analysis of the feasibility of reversible computing is performed. The key question is: Is it possible to build a completely reversible computer? A closer look into the internal aspects of the reversible computing as well as the external constraints such as the second law of thermodynamics has demonstrated that several difficulties would have to be solved before reversible computer is...

Covalent inhibition is a reemerging paradigm in kinase drug design, but the roles of inhibitor binding affinity and chemical reactivity in overall potency are not well-understood. To characterize the underlying molecular processes at a microscopic level and determine the appropriate kinetic constants, specialized experimental design and advanced numerical integration of differential equations a...

It is a classical result from universal algebra that the notions of polymorphisms and invariants provide a Galois connection between suitably closed classes (clones) of finitary operations f : B n → B, and classes (coclones) of relations r ⊆ B k. We will present a generalization of this duality to classes of (multi-valued, partial) functions f : B n → B m , employing invariants valued in partia...

We report two organocatalysts for CO2 hydroboration to methylborylethers, which upon hydrolysis can produce methanol. These organocatalysts feature carbon-centered reversible CO2 binding, broad borane scopes, and high catalytic activities.

The movement of a copper ion inside the cavity of cryptands having two distinct metal binding sites at each end is linked to a fluorescence ON-OFF process in a reversible manner, providing a new way of fluorescence switching.