نتایج جستجو برای: reverse transcriptase enzyme

تعداد نتایج: 344010  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2017
Carrie Arnold

Adaptation, a cornerstone of evolutionary change, is rarely straightforward. Acquiring a random mutation that promotes survival can take generations. Prokaryotes such as bacteria and Archaea, along with the viruses they harbor, have compact genomes, leaving them with a limited repertoire of DNA to respond to environmental change. Fifteen years ago, microbiologist Jeffrey Miller at the Universit...

Journal: :Bioscience, biotechnology, and biochemistry 2010
Masaki Mizuno Kiyoshi Yasukawa Kuniyo Inouye

We explored the mechanism of the stabilization of Moloney murine leukaemia virus reverse transcriptase (MMLV RT) by eliminating RNase H activity. Without the template-primer (T/P) poly(rA)-p(dT)(15), the temperature reducing initial reverse-transcription activity by 50% over a 10-min incubation of the RNase H activity-deficient variant D524A was higher by 3.7 degrees C than that of the wild-typ...

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