the objective of the current study was to evaluate the reproducibility of a reverse transcriptase pcr (rt-pcr)-based technique to differentiate viable and dead salmonella cells in raw and sterilized milk. the microorganism was initially inoculated into the milk samples followed by incubating at 37°c for 4 h prior to inactivation by heat at 80°c for 10 min. the treated and non-treated samples we...

Aortic stiffness, peripheral wave reflection, and aorta-to-peripheral pulse pressure amplification all predict cardiovascular risk. However, the pathophysiological mechanism behind it is unknown. Tonometric pressure waveforms were recorded on the radial, carotid, and femoral arteries in 138 hypertensive patients (age: 56±13 years) to estimate aorta-to-peripheral amplifications, aortic augmentat...

Mitochondria generate reactive oxygen species (ROS) dependent on substrate conditions, O(2) concentration, redox state, and activity of the mitochondrial complexes. It is well known that the FADH(2)-linked substrate succinate induces reverse electron flow to complex I of the electron transport chain and that this process generates superoxide (O(2)(*-)); these effects are blocked by the complex ...

Across cultures, people conceptualize time as if it flows along a horizontal timeline, but the direction of this implicit timeline is culture-specific: in cultures with left-to-right orthography (e.g., English-speaking cultures) time appears to flow rightward, but in cultures with right-to-left orthography (e.g., Arabic-speaking cultures) time flows leftward. Can orthography influence implicit ...

Different CFD models of reverse-flow centrifugal separators namely swirl tubes have been built in order to study and detail analysis of the phenomenon of the "end of the vortex". The present work based on previous experience and experimental activities in the field of this phenomenon. The models was built in absolutely agree with geometrical characteristics and operating conditions of experimen...

BACKGROUND Competitive PCR of reverse transcribed mRNA sequences is used to quantify transcripts, but the usual approaches are labor-intensive and time-consuming. We describe the non-gel-based quantification of competitive reverse transcription (RT)-PCR products with use of microparticles and flow cytometry. METHODS PCR products of a target sequence and an internal control sequence (competito...

BACKGROUND The scapular osteocutaneous flap can be used for the reconstruction of a variety of head and neck defects. In this study, we describe use of the reverse-flow scapular osteocutaneous flap in the setting of a vessel-depleted neck. METHODS AND RESULTS A retrospective review of patients who underwent scapular osteocutaneous free flap reconstruction over a 5-year period revealed 3 patie...

The behavior of a reverse-flow reactor was studied for the purification of polluted air by catalytic combustion. A heterogeneous one-dimensional model was extended with a heat balance for the reactor wall. An overall heat transport term is included to account for the small heat losses in radial direction. The calculations are compared to experimental data without using fit parameters. The agree...

The global flow of knowledge, skills, and ideas has been a defining feature of human progress. Different regions and peoples have contributed to and, indeed, led innovation development at various times in human history. From Africa to Asia, Latin America, and the Middle East, the current body of knowledge on these diverse contributions to human science and medicine is expanding [1]. For example...

The deep venous system of the upper and lower extremities was injected with a lead oxide mixture in 2 fresh human cadavers, dissected, radiographed and the sites of the venous valves located. These studies confirmed that the macrovenous connections between the venae comitantes of the distributing arteries were insufficient in number to bypass the venous valves in conventional, distally based re...