PURPOSE Angiogenesis accompanies several retinal pathologies that impair the inner blood-retinal barrier. Claudins are key structural and functional proteins of the barrier. This study compared the expression of claudins during the normal angiogenesis of development with that of oxygen-induced retinopathy. METHODS Real-time PCR was used to monitor mRNA from postnatal day 8 (P8) to P21 in norm...

BACKGROUND The morphological changes of the retinal blood vessels in retinal images are important indicators for diseases like diabetes, hypertension and glaucoma. Thus the accurate segmentation of blood vessel is of diagnostic value. METHODS In this paper, we present a novel method to segment retinal blood vessels to overcome the variations in contrast of large and thin vessels. This method ...

We demonstrate in vivo measurements in human retinal vessels of an experimental parameter, the slope of the low coherence interferometry (LCI) depth reflectivity profile, which strongly correlates with the real value of blood hematocrit. A novel instrument that combines two technologies, spectral domain low coherence interferometry (SDLCI) and retinal tracking, has been developed and used for t...

OBJECTIVE Infantile hemangioma (IH) is a rapidly growing vascular tumor affecting newborns. It is composed of immature endothelial cells and pericytes that proliferate into a disorganized mass of blood vessels. We isolated pericytes from IH (Hem-pericytes) to test our hypothesis that Hem-pericytes are unable to stabilize blood vessels. METHODS AND RESULTS We injected pericytes in vivo, in com...

PURPOSE To develop a sensitive and reproducible technique for measuring the adherence of blood lymphocytes to vessel walls exposed in sections of human retina and for examining the role of lymphocyte and vascular adhesion molecules in these events. METHODS Cryostat sections of human retina were overlaid with blood lymphocytes from healthy subjects, and experimental conditions were sought by w...

Diabetic Retinopathy is a effect of diabetes. It results abnormalities in the retinal blood vessels. The can cause blurry vision and blindness. Automatic vessels segmentation on image detect these vessels, actually resulting faster more accurate results. paper proposed an automatic vessel method that combined Otsu Thresholding with enhancement techniques. In enhancement, it CLAHE Top-hat transf...

Ocular fundus image assessment has been extensively used by ophthalmologists for diagnosing vascular and non vascular pathology. Examining the retinal blood vessel network may reveal arteriosclerosis, diabetes, hypertension, cardiovascular disease and stroke [12]. Furthermore, the segmentation of the vessel network is the most suitable representation for the retinal image registration since vas...

Retinal blood vessels segmentation plays an important role for retinal image analysis. In this paper, we propose robust retinal blood vessel segmentation method based on reinforcement local descriptions. A novel line set based feature is firstly developed to capture local shape information of vessels by employing the length prior of vessels, which is robust to intensity variety. After that, loc...

PURPOSE To quantitatively model the changes in blood velocity profiles for different cardiac phases in human retinal vessels. METHODS An adaptive optics scanning laser ophthalmoscope (AOSLO) was used to measure blood velocity profiles in three healthy subjects. Blood velocity was measured by tracking erythrocytes moving across a scanning line. From the radial position of the cells within the ...