NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE (http://www.neb.com/rebas...

Type I DNA restriction enzymes are large, molecular machines possessing DNA methyltransferase, ATPase, DNA translocase and endonuclease activities. The ATPase, DNA translocase and endonuclease activities are specified by the restriction (R) subunit of the enzyme. We demonstrate that the R subunit of the Eco KI type I restriction enzyme comprises several different functional domains. An N-termin...

the pathogenicity of a recent isolate of infectious bursal disease virus (ibdv), ir499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible spf chickens, chickens embryos and broilers was discussed. the molecular characterization of the virus based on the restriction fragment length polymorphism (rflp) pattern was also investigated. the mortality rates were 85% and 22%...

Abstract Background Emergence of fluoroquinolone resistance in gut pathogens is a cause concern. Resistant to quinolone mainly due the point mutations at quinolone-resistance determining regions (QRDR). The aim study was develop polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) detect QRDR gyrA and gyrB enteric pathogens. Methodology PCR-RFLP done for 83 region...

To our knowledge, although comparison of chromosomic macrorestriction patterns has become one of the most feasible molecular tools of the current microbial taxonomy, a mathematical approach to optimize the choice of a restriction enzyme among the endonucleases tested for such comparison has not been previously described. The coincidence of restriction patterns for two tested bacterial strains w...

A sensitive assay for O6-alkylguanine-DNA alkyltransferase activity in cell or tumor extracts has been devised. The theoretical basis of the new assay lies in the observation that certain restriction enzymes will not cleave DNA containing methylated bases. Thus, if a synthetic oligodeoxynucleotide with a restriction sequence containing O6-methylguanine were incubated with the restriction enzyme...

background and objectives: to investigate coagulase gene polymorphisms of mrsa and mssa isolates from shiraz teaching hospitals from 2011 to 2012. materials and methods: a total of 302 isolates of staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. the isolates were subj...

Nicotiana tabacum chloroplast DNA contains two copies each of 16S and 23S rRNA genes. These genes are located in an inverted order as determined from restriction fragment mapping and Southern hybridization to restriction fragments. The position of these genes on the N. tabacum chloroplast DNA molecule has been established relative to a complete map of SalI and SMaI restriction enzyme cleavage s...

The DNAs of a varicella-zoster virus vaccine and its parental virus were compared by CsCl buoyant density centrifugation and restriction enzyme cleavage analysis. The varicella-zoster virus vaccine DNA showed a heterogeneous buoyant profile and altered restriction enzyme cleavage patterns. These changed properties are probably the result of the accumulation of virus containing defective varicel...