The mouse dihydrofolate reductase (dhfr) gene possesses a bidirectional promoter that produces functional transcripts in the opposite direction. These opposite strand transcripts encode the Rep-3 gene product, a protein that has homology to DNA mismatch repair enzymes. The core of the bidirectional promoter consists of four consensus binding sites for the transcription factor Sp1. These binding...

Genome duplication requires accessory helicases to displace proteins ahead of advancing replication forks. Escherichia coli contains three helicases, Rep, UvrD and DinG, that might promote replication of protein-bound DNA. One of these helicases, Rep, also interacts with the replicative helicase DnaB. We demonstrate that Rep is the only putative accessory helicase whose absence results in an in...

The Rochester Epidemiology Project (REP) is a unique research infrastructure in which the medical records of virtually all persons residing in Olmsted County, Minnesota, for over 40 years have been linked and archived. In the present article, the authors describe how the REP links medical records from multiple health care institutions to specific individuals and how residency is confirmed over ...

To examine the coupling of ATP hydrolysis to helicase translocation along DNA, we have purified and characterized complexes of the Escherichia coli Rep protein, a dimeric DNA helicase, covalently crosslinked to a single-stranded hexadecameric oligodeoxynucleotide (S). Crosslinked Rep monomers (PS) as well as singly ligated (P2S) and doubly ligated (P2S2) Rep dimers were characterized. The equil...

We advance a quantitative description of the critical shear rate γ̇c needed to dislodge a spherical particle resting on a surface with a model asperity in laminar and turbulent fluid flows. We have built a cone-plane experimental apparatus which enables measurement of γ̇c over a wide range of particle Reynolds number Rep from 10−3 to 1.5 × 103. The condition to dislodge the particle is found to b...