Ionotropic GABA receptors are the targets for several classes of insecticides. One of the most widely-studied insect GABA receptors is RDL (resistance to dieldrin), originally isolated from Drosophila melanogaster. RDL undergoes alternative splicing and RNA editing, which influence the potency of GABA. Most work has focussed on minority isoforms. Here, we report the first characterisation of th...

Stability of three batches of freeze dried inactivated mouse brain Japanese encephalitis vaccine, was evaluated in terms of percentage drop or otherwise in potency from their respective initial titers, after exposure to different experimental conditions for 4 -12 weeks. The potency was assayed by plaque reduction neutralization assay (PRNT) using chick embryo fibroblasts cells. The vaccine has ...

A dioxinlike compound is a compound that binds to the aryl hydrocarbon (Ah) receptor, results in dioxinlike effects, and bioaccumulates. These are the three factors for including dioxinlike chemicals in the toxic equivalency factor (TEF) concept. Risk assessment of dioxinlike compounds is based on using these TEFs. Hexachlorobenzene (HCB) has all three features and should therefore be included ...

measles has been a major cause of illness and death in children and vaccination against the disease is part of the who global immunization program. a suitable vaccine should create maximum immune response against the pathogen and must be safe for the user. thus, after production, vaccines must be analyzed and controlled by the producer and confirm by relevant governmental organizations. the foo...

poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. for accurate vaccine evaluation, three types of cell lines including hela, hep2c and vero were evaluated and compared using two methods of...

397 Standardization and Characterization of Dust Mite Extracts Manufactured in the USA Greg Plunkett, PhD. ALK-Abello, Round Rock, TX. Background: Standardized Dermatophagoides dust mite extracts are produced in the US from purified whole bodies. Growth media and the processes for separating mites from media vary among manufacturers. The FDA requires that mite extracts are standardized and labe...

397 Standardization and Characterization of Dust Mite Extracts Manufactured in the USA Greg Plunkett, PhD. ALK-Abello, Round Rock, TX. Background: Standardized Dermatophagoides dust mite extracts are produced in the US from purified whole bodies. Growth media and the processes for separating mites from media vary among manufacturers. The FDA requires that mite extracts are standardized and labe...

BACKGROUND Buame [17β-(butylamino)-1,3,5(10)-estratrien-3-ol] possesses anticoagulant and antiplatelet activities that are potentially antithrombotic. Since its estrogenicity is unknown, it was evaluated by established methods. METHODS Buame (10, 100, 500, and 1,000 μg/kg), 17β-estradiol (E(2)) (100 μg/kg), or propylene glycol (10 ml/kg) were subcutaneously (sc) administered for three days to...

397 Standardization and Characterization of Dust Mite Extracts Manufactured in the USA Greg Plunkett, PhD. ALK-Abello, Round Rock, TX. Background: Standardized Dermatophagoides dust mite extracts are produced in the US from purified whole bodies. Growth media and the processes for separating mites from media vary among manufacturers. The FDA requires that mite extracts are standardized and labe...

Geldanamycin (GDM) has been modified by different type neutral/acidic/basic substituents (1–7) and quinuclidine motif (8), transformed into ammonium salts (9–13) at C(17). These compounds have characterised spectroscopic x-ray methods. Derivative 8 shows better potency than GDM in MCF-7, MDA-MB-231, A549 HeLa (IC50s = 0.09–1.06 µM). Transformation of 9–13 reduces toxicity (by 11-fold) attractiv...