Snapin, a 15-kDa protein, has been identified recently as a binding partner of SNAP-25. Moreover, snapin is regulated by phosphorylation and enhances synaptotagmin binding to SNAREs. Furthermore, snapin and C-terminal snapin fragments have been effective in changing the release properties of neurons and chromaffin cells. Here we have reinvestigated the role of snapin using both biochemical and ...

In diseases such as diphtheria and tetanus, almost all the clinical symptoms terminating in deat.h are due to specific soluble toxins produced in civo (or in vitro) by the diphtheria and t&anus bacilli respectively. Although diphtheria toxin has been isolated as an essentially pure protein which has been reasonably well characterized both chemically and physically (l-3), almost’ nothing is know...

In buffer containing 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 5 mM imidazole, pH 7.5, 0.1 mM CaCl2, 0.2 mM dithiothreitol, 0.01% NaN3, and 0.2 mM ATP, the KD for the formation of the 1:1 complex between Acanthamoeba actin and Acanthamoeba profilin was about 5 microM. When the actin was modified by addition of a pyrenyl group to cysteine 374, the KD increased to about 40 microM but the critical concent...

I 4 ¯ -carbon was first proposed by Zhang et al., this paper will report regarding this phase of carbon. The present paper reports the structural and elastic properties of the three-dimensional carbon allotrope I 4 ¯ -carbon using first-principles density functional theory. The related enthalpy, elastic constants, and phonon spectra confirm that the newly-predicted I 4 ¯ -carbon is thermodynami...

Abstract. A reinvestigation of the type angastonite specimen from Penrice marble quarry, South Australia, shows that published powder X-ray diffraction pattern for mineral corresponds to a mixture crystalline phases plus an amorphous phase. The formula mineral, CaMgAl2(PO4)2(OH)4⋅7H2O, has been found correspond proposal redefinition as phase approved by IMA CNMNC. Amorphous is isotropic with in...